EB病毒感染IM患儿血浆sHLA-G水平与外周血淋巴细胞亚群关系研究

The research on the relationship between plasma s HLA-G level and peripheral blood lymphocyte subsets of the children with IM caused by EBV infection

目的观察和分析Epstein-Barr病毒(EBV)感染传染性单核细胞增多症(IM)患儿血浆可溶性人类白细胞抗原G(s HLA-G)水平与外周血淋巴细胞亚群的关系。方法选取90例EBV感染IM患儿作为病例组,选取90例健康儿童作为对照组,对两组患者血浆s HLA-G水平和外周血淋巴细胞亚群进行检测和比较。结果病例组患儿的血浆s HLA-G水平显著高于对照组儿童(Z=59.128,P<0.05);病例组患儿的外周血CD3+淋巴细胞、CD8+淋巴细胞的百分比均显著高于对照组(t=10.365、19.205,P<0.05),而其外周血CD4+淋巴细胞、CD16+56+淋巴细胞、CD19+淋巴细胞的百分比及CD4+/CD8+淋巴细胞比值均显著低于对照组(t=12.183、4.358、8.138、10.226,P<0.05);患儿血浆s HLA-G水平与其外周血淋巴细胞亚群指标均无相关性(P>0.05)。结论 EBV感染IM患儿表现为血浆s HLA-G高表达和外周血淋巴细胞亚群紊乱,患儿机体内存在着明显的免疫耐受和免疫抑制,但s HLA-G高表达并不是引起患儿免疫功能下降的直接原因。

Objective：To observation and analyze the relationship between plasma soluble human leukocyte antigen-G（sHLA-G）level and peripheral blood lymphocyte subsets of the children with infectious mononucleosis（IM）caused by Epstein-Barr virus（EBV）infection. Methods：90 cases of children with IM caused by EBV infection were selected as the case group and 90 healthy children were selected as the control group. The plasma sHLA-G level and the peripheral blood lymphocyte subsets of the children in the two groups were detected and compared. Results：The plasma sHLA-G level of the children in the case group was significantly higher than that of the control group（Z=59.128,P〈0.05）;The percentages of peripheral blood CD3＋ lymphocytes,CD8＋ lymphocytes of the children in the case groups were significantly higher than those in the control group（t=10.365,19.205,P〈0.05）,while the ratio of CD4＋/CD8＋ lymphocytes and the percentages of peripheral blood CD4＋ lymphocytes,CD16＋56＋ lymphocytes,CD19＋ lymphocytes were significantly lower than those of the control group（t=12.183,4.358,8.138,10.226,P〈0.05）;There was no correlation between plasma sHLA-G level and the indexes of peripheral blood lymphocyte subsets of the children（P〉0.05）. Conclusions：The children with IM caused by EBV infection showed high expression of plasma sHLA-G and the disorder of peripheral blood lymphocyte subsets. There are significant immune tolerance and immune inhibition in their body but high expression of sHLA-G is not the direct cause of the decline of the immune function of the children.