猪伪狂犬病毒、副猪嗜血杆菌和猪链球菌混合感染的病原分离与鉴定

Pathogen Isolation and Identification of Mixed Infection of Pseudorabies Virus,Haemophilus parasuis and Streptococcus suis

为了探究河南省某规模化猪场育肥猪发生疫情的病因,进行了猪伪狂犬病毒的血清学和PCR鉴别诊断,同时对分离细菌进行了形态鉴定、PCR鉴定、血清型鉴定和药敏试验。结果显示,猪伪狂犬野毒血清学和PCR检测均为阳性,同时分离到副猪嗜血杆菌和猪链球菌。经鉴定副猪嗜血杆菌为血清4型,链球菌为血清2型。药敏试验结果表明,副猪嗜血杆菌和链球菌对头孢哌酮、头孢他定、头孢噻肟、头孢曲松、头孢呋辛、头孢唑啉、亚胺培南、阿莫西林、氨苄西林、万古霉素敏感。

In order to explore the pathogen of outbreak of epdemic of fattening pigs in a large-scale pig farm in Henan province,the serology and PCR differential diagnosis of wild pseudorabies virus（PRV）, morphology and PCR identification,serotype and drug sensitive test of bacteria were carried out.Results showed that the serology and PCR differential diagnosis of wild PRV were positive,Haemophilus parasuis （HPS） and Streptococcus suis（SS） were isolated.After identification HPS was serotype 4 and SS was serotype 2.Drug sensitive test showed that HPS and SS were sensitive to cefoperazone, ceftazidime, cefotaxime,ceftriaxone,cefuroxime,cefazolin,imipenem,amoxicillin,ampicillin and vancomycin.