摘要
目的探索腺相关病毒介导的IFNα体外抑制HBV的效果,寻求治疗HBV感染的新策略。方法 3种质粒共转染制备重组病毒AAV-IFNα,用AAV-IFNα感染肝细胞系HepG2.2.15,感染后1、3、6和9 d,q PCR检测HBV DNA;ELISA方法检测HBs Ag和HBe Ag含量;MTT法检测Hep G2.2.15细胞增殖的情况。结果 AAV-IFNα可通过抑制HepG2.2.15细胞增殖的方式显著抑制HBV的复制与表达(P<0.05)。结论 AAV-IFNα是一种有潜力的抗HBV的药物。
Objective To identify the anti-HBV efficiency of AAV-IFNα by infecting HepG2. 2. 15 cell line, and to develop a new strategy of curing hepatitis B. Methods Firstly,recombinant vector was packaged into AAV by three plasmids co-transfection method ; then AAV-IFNα infected HepG2. 2. 15 cell line, and samples were collected on the 1st, 3rd,6th and 9th day; thirdly, The time-course of HBV DNA,HBsAg and HBeAg ELISA. The proliferation of HepG2. 2. 15 cells detected by MTr. Results Recombinant inhibit HBV replication and expression in vitro because of the proliferation of HepG2. 2. IFNc^（P 〈 0. 05）. Conclusions AAV-IFNe~ is a potential antiviral product for hepatitis were detected by qPCR and AAV-IFNα can significantly 15 cells inhibited by AAV6- B.
出处
《基础医学与临床》
CSCD
2015年第10期1331-1335,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(51402027)
关键词
乙型肝病毒
腺相关病毒
干扰素
hepatitis B virus
adeno-associated virus
interfron
