舞毒蛾羧酸酯酶最佳反应体系的确立

Establishment of Optimum Reaction System of Carboxylesterase from Gypsy Moth(Lymantria dispar)

为了确定舞毒蛾(Lymantria dispar)羧酸酯酶(Car E)活性的最佳反应体系,运用正交试验测定了反应体系中酶浓度、底物浓度、p H值、反应温度和反应时间5个因素对Car E活性的影响。极差分析和方差分析显示,舞毒蛾Car E的最优反应体系为酶浓度0.2头·m L-1,底物浓度0.2 mmol·L-1,p H=7.5,反应温度45℃,反应时间10 min。

In order to study the optimum reaction system of carboxylesterase （CarE） from the gypsy moth （Lymantria dispar）, we used orthogonal experiment to measure the effects of the concentrations of carboxylesterase and substrate, pH, reaction temperature and reaction time to the activity of CarE. Through analyzing the data from orthogonal experiment with range analy- sis and analysis of variance, the optimum reaction system of carboxylesterase was ultimately determined as 0.2 beetle · mL-1 of carboxylesterase concentration, 0.2 mmol· L-1 of substrate concentration, 7.5 of pH, 45 ℃ of reaction temperature, and 10 min of reaction time.