原发性干燥综合征患者体内维生素D3水平评估及其与病变程度、免疫功能的关系

Evaluation of vitamin D3 levels in primary Sjogren's syndrome patients and its correlation with severity and immune function

目的:研究原发性干燥综合征患者体内维生素D3水平评估及其与病变程度、免疫功能的关系。方法:选择活动期pSS患者、稳定期pSS患者以及健康志愿者进行研究,采集外周血、分离单个核细胞并检测CD27high浆细胞以及CD27+记忆性B细胞所占比例;采集血清并检测1,25(OH)2D3、TNF-α、IL-6、IL-10、IL-17含量。结果:(1)pSS组血清中1,25(OH)2D3含量以及外周血中CD27high浆细胞、CD27+记忆性B细胞的含量低于对照组,差异有统计学意义(P<0.05);血清中TNF-α、IL-6、IL-17含量高于对照组,IL-10含量低于对照组,差异有统计学意义(P<0.05);(2)活动期pSS患者血清中1,25(OH)2D3含量以及外周血中CD27high浆细胞、CD27+记忆性B细胞的含量低于稳定期pSS患者,差异有统计学意义(P<0.05);血清中TNF-α、IL-6、IL-17含量高于稳定期pSS患者,IL-10含量低于稳定期pSS患者,差异有统计学意义(P<0.05);(3)1,25(OH)2D3含量与CD27high浆细胞、CD27+记忆性B细胞以及IL-10的含量呈正相关,与TNF-α、IL-6、IL-17含量呈负相关。结论:维生素D3水平异常降参与原发性干燥综合征的发病,与疾病的病变程度、免疫功能密切相关。

Objective： To study the vitamin D3 levels in primary Sjogren＇s syndrome （pSS） patients and its correlation with severity and immune function. Methods： Active pSS patients, stable pSS patients and healthy volunteers were chosen for study. Peripheral blood was collected, mononuclear cells were isolated and percentages of CD27high plasma cells and CD27^＋ memory B cells were detected; serum was collected and contents of 1,25（OH）2D3, TNF-α, IL-6, IL-10 and IL-17 were detected. Results： （1） 1,25（OH）2D3 contents in serum as well as CD27^high plasma cell and CD27^＋ memory B cell contents in pe ripheral blood of pSS group were significantly lower than those of control group （P〈0.05） ; serum TNF-α, IL-6 and IL 17 contents were significantly higher than those of control group （P〈0.05）and IL-10 contents were significantly lower than those of control group （P〈0.05）; （2） 1,25（OH）2D3 contents in serum as well as CD27^high plasma cell and CD27^＋ memory B cell contents in peripheral blood of active pSS patients were significantly lower than those of stable pSS patients （P〈0.05） ; serum TNF-α, IL-6 and IL-17 contents were significantly higher than those of stable pSS patients （P〈0.05）and IL-10 contents were