微生态制剂联合锌制剂治疗小儿迁延性腹泻病后血清及结肠灌洗液相关指标的评价

Change of serum and colon lavage fluid related indicators after probiotics combined with zinc preparations treatment for persistent diarrhea in children

目的:探究微生态制剂联合锌制剂治疗迁延性腹泻患儿后血清及结肠灌洗液相关指标的表达变化。方法:选取本院诊治的80例腹泻儿童,根据临床症状、血常规及大便检查均确诊为迁延性腹泻,将患儿随机分成对照组和实验组,各40例,对照组采用基础治疗和锌制剂(葡萄糖酸锌片)治疗,实验组在对照组基础上采用微生态制剂(思连康)联合锌制剂治疗,收集两组治疗前后空腹血清及结肠灌洗液,检测白介素-6(IL-6)、白介素-8(IL-8)、一氧化氮(NO)、丙二醛(MDA)及超氧化物歧化酶(SOD)表达水平,比较两组间的差异。结果:两组经治疗后,血清及结肠灌洗液IL-6、IL-8表达水平较治疗前均明显下降,实验组下降更为明显,差异具有统计学意义(P<0.05);两组血清及结肠灌洗液NO、MDA及SOD表达水平经治疗后均较治疗前明显好转,实验组较对照组更接近正常水平,差异具有统计学意义(P<0.05)。结论:采用微生态制剂联合锌制剂治疗小儿迁延性腹泻疗效明显,有效下调血清和结肠灌洗液炎症因子及氧化应激因子的表达水平,值得在今后的临床治疗中推广应用。

Objective： To explore the expression change of serum and colon lavage fluid related indicators after probiot- ics combined with zinc preparations treatment for persistent diarrhea in children. Methods： A total of 80 children with diarrhea treated in our hospital were selected. According to clinical symptoms, routine blood and stool test, they were all diagnosed with persistent diarrhea and randomly divided into control group and experimental group, with 40 cases in each group. Control group received basic treatment and zinc preparations （zinc gluconate tablets） therapy; experimental group, based on treatment of control group, received probiotics （Siliankang） combined with zinc preparations treatment. Fasting serum and colon lavage fluid of both groups were collected before and after treatment to detect IL-6, IL-8, NO, MDA and SOD expression levels, and differences between two groups were compared. Results： After treatment, IL-6 and IL-8 expression levels in serum and colon lavage fluid of both groups decreased more significantly than those before treatment （P〈0.05）, and those of experimental group decreased more significantly （P〈0.05）; after treatment, NO, MDA and SOD expression levels in serum and colon lay-age fluid of both groups were more significantly improved than those before treatment （P〈0.05）, and those of experimental group were closer to the normal levels than those of control group （P〈0. 05）. Conclusions： Probiotics combined with zinc preparations treatment of persistent diarrhea in children has obvious curative effect; it effectively reduces expression levels of inflammatory cytokines and oxidative stress factors in serum and colon lavage fluid, and is worth popularization in future clinical treatment.