旋毛虫A200711蛋白对裸鼠H7402细胞实体瘤的抑制作用

Inhibitory effect of the A200711 protein fromtrichinella spiralis on BALB/c nude mice bearing H7402 solid tumor

为了探讨旋毛虫A200711蛋白对BALB/c裸鼠皮下人肝癌H7402细胞实体瘤的抑制作用。本试验将BALB/c裸鼠右侧腋下注射人肝癌H7402细胞,建立裸鼠实体瘤模型。成瘤后,每2d向实体瘤中间部位多点注射0.2mL质量浓度为7.5mg/L的旋毛虫A200711蛋白,观察裸鼠一般临床症状及实体瘤生长情况;每3d称量裸鼠体质量,测量肿瘤直径,计算肿瘤的体积,绘制肿瘤生长曲线;3周后处死裸鼠,小心剥离肿瘤组织,称量肿瘤的重量,计算抑瘤率。20d后裸鼠腋部皮下均长出肿瘤结节,成瘤率为100%,平均直径3~5 mm;与模型对照组比较,注射A200711蛋白12d后裸鼠实体瘤体积明显缩小,抑瘤率达到39.67%。结果表明,旋毛虫A200711蛋白对裸鼠人肝癌细胞实体瘤的生长具有明显的抑制作用。

To investigatethe inhibitory effect on BALB/c nude mice bearing the human H7402 solid tumor by A200711 protein from trichinella spiralis. Human hepatocellular carcinoma H7402 cells were grafted on the back of BALB/c nude mice. After bearing tumor,the bearing tumor mice were treated with 7.5 mg/L A200711 protein every two days. The growth,diameter of solid tumor and weight of tumor bearing mice were recorded to calculate the tumor volume and draw the tumor growth curve every 3 days. After 3 weeks, the tumor bearing mice were sacrificed. Tumors were harvested,weighed and then calculated the inhibitory rate. After 20 days, all experimental nude mice beared tumors. The tumor grafted rate was 100% ,the average diameter was 3-5 mm;Com- pared with the model control group,the solid tumor volume of nude mice treated with the A200711 protein for 12 days were drastically shrunk and the inhibitory rate reached 39.67%. A200711 pro- tein from trichinella spiralis significantly inhibit the expansion of human hepatocellular carcinoma H7402 cells in nude mice.