辽宁地区奶牛临床型乳房炎病原菌的分离鉴定及耐药性分析

Isolation,identification and drug sensitivity test of main pathogenic bacteria caused dairy cow mastitis in Liaoning region

为了获得辽宁地区奶牛临床型乳房炎病原菌及其药物敏感性的系统资料,为指导临床合理用药提供依据,本试验从辽宁地区的奶牛场采集临床型乳样100份,然后对乳样进行微生物常规分离、采用MicroScan AutoScan-4型自动微生物鉴定及药物敏感性测定仪进行检测,分离到24种细菌共227个菌株,其中金黄色葡萄球菌66株,占29.07%;大肠杆菌46株,占20.26%。可见引起辽宁省奶牛临床型乳房炎的病原菌以金黄色葡萄球菌和大肠杆菌为主,而且以混合感染较为严重。药敏分析结果表明,头孢唑啉和庆大霉素对临床型乳房炎的主要致病菌的抑制效果很好,主要致病菌对青霉素产生完全耐药性。

The aim of this study was to analyze the characteristics of the pathogenic bacteria caused dairy cow mastitis,and to provide a theorical foundation for clinical administration, cure and pre- vention. The appraisal and drug sensitivity to isolated bacteria were analysed by MicroScan Auto- Scan-4. 227 strains of bacteria were isolated from the 100 milk samples of dairy cows with clinical mastitiS in Liaoning area. Result showed that 66 strains among them are Staphylococcus aureus （29.07 %） ,46 are Escherichia coli （20.26 %）. Drug sensitivity test showed that all pathogenic bac- teria are resistant to penicillin, while cefazolin and gentamycin have a good inhibiting effect on these main pathogenic bacteria.