高血压脑出血模型大鼠不同时间段脑损伤程度与NO含量和C9表达的关系

Analysis of Cerebral Tissue Damage and Expression of C9 and NO at Different Time of Rats after Hypertensive Intracerebral Hemorrhage

目的研究高血压脑出血模型大鼠不同时间段脑损伤程度与脑组织NO含量和C9表达的关系。方法采用胶原酶法制备脑出血动物模型,将96只大鼠随机分为均等的3部分（每部分32只）,分别用于脑组织含水量测定、脑组织NO水平测定和C9表达检测。每部分又随机分为假手术组、模型组共2组,每组16只;每个小组16只大鼠再4个为一组,于造模后2、12、24、72h测各组大鼠脑组织含水量,脑组织C9表达,NO含量。结果与假手术组比较,模型组24~72h脑组织含水量明显增多、脑组织C9表达增多、NO含量减少。结论脑出血急性期的脑组织损伤程度与脑组织NO水平、脑组织C9表达有一定关系,临床治疗中改善脑循环、减轻炎症反应非常重要。

Objective To investigate cerebral tissue damage degree and the expression of complement C9 and NO in rats at different time after hypertensive intracerebral hemorrhage （ ICH ） .Methods Rat ICH model was established by Collagenase infusion .96 rats were divided into 3 groups, 32 rats in each group.Each group was randomly subdivided into sham group and model group .Brain water content and the levels of nitric oxide （NO） and C9 were determined at 2h,12h, 24h and 72h after ICH.Results Brain water contents and expression of C9 in model group were increased remarkably 24 to 72 hours after operations , while the content of NO was decreased as compared to sham group .Conclusion Cerebral tissue damage degree in acute intracerebral hemorrhage was associated with content of NO , expression of C9 in rats cerebral tissues.It＇s important to improve brain circulation and alleviate inflammatory response .