Enterabactersp．CV—b对结晶紫的脱色特性研究

Exploration on Characteristics of the Decolorization of Crystal Violet by Enterabacter sp. CV-b

结晶紫是一类难以降解且对许多生物都具有致癌致畸性的三苯甲烷类染料。从浙江温州分离筛选到1株结晶紫高效降解菌株CV—b，经16SrRNA基因序列分析表明，该菌株属于Enterabactersp．属。当pH值在3．0～10．0之间时，培养24h以后，该菌株对50mg／L结晶紫的脱色率均在50％以上；脱色的最适温度范围为25～35℃之间；多数供试碳源对脱色有显著促进效应，而多数供试氮源则对脱色无显著影响；供试金属离子中。Cu^2＋、Fe^3＋和Zn^2＋对脱色有显著的促进效应。动力学实验结果表明。该菌株对结晶紫的脱色与对数模型拟合度较高（R2=0．9427）。经脱色前后的全波长扫描分析显示，菌株CV—b对结晶紫的脱色是由生物降解引起的。总体而言，菌株CV—b在结晶紫脱色中的实际应用潜能较大。

Crystal Violet （CV） is a recalcitrant triphenylmethane dye which is carcinogenic and mutagenie against many organisms. The strain CV-b capable of degrading CV efficiently was isolated from Wenzhou, Zhejiang. Results of 16S rRNA gene analysis showed that the strain is a member of Enterabacter sp. The CV decolorization by the strain after 24 h at pH 3.0-10.0 could reach over 50%. The optimal temperature for decolorization is between 25-35℃. Most of the tested carbon resources could significantly enhance the decolorization, while most of the tested nitrogen resources have no significant effect on decolorization. Among the tested metal ions, Cu^2＋,Fe^3＋ and Zn^2＋ could significantly enhance the decolorization. Results of kinetics studies indicated that the data of kinetics fitted the logarithmic model well （R2 = 0.9427）. The UV-visible spectrum indicated that the CV decolorization by the strain CV-b was due to the biodegradation. In general, the strain CV-b will have a great potential application in CV degradation.