摘要
目的观察复方二黄颗粒对慢性肾衰竭大鼠的肾组织病理及TGF-β1(转化生长因子-β1)、FN(纤维连接蛋白)、α-SMA(α-平滑肌肌动蛋白)表达的影响。方法成年雄性SD大鼠30只,随机分为假手术组、模型对照组、治疗组共3组;5/6肾切除喂养4周后,各组大鼠分别给予药物及等量生理盐水灌胃共8周后,处死所有大鼠,留取肾组织。观察各组大鼠的肾脏病理变化,并用免疫组化法观察肾组织TGF-β1、FN、α-SMA的表达,并进行统计学分析。结果复方二黄颗粒能改善肾衰大鼠的肾小球硬化、间质纤维化;并能抑制肾衰大鼠肾组织中TGF-β1、FN、α-SMA表达的上调,各组间比较有统计学意义(P<0.05)。结论复方二黄颗粒能改善慢性肾衰竭大鼠的肾脏纤维化。
Objective To observe the effects of Erhuang compound granule on protecting and curing chronic renal failure (CRF)due to 5 /6 nephrectomy (NPX).Methods Thirty rats with 5 /6 nephrectomy were assigned into 3 groups:nonoperation group,excision mod-el group and Erhuang compound granule group.Then,each group was fed with different medicines for 8 weeks before the rats were killed and the kidneys were used for pathological testing.Results Erhuang compound granule could significantly down -regulate protein ex-pressions of TGF-β1 ,FN and α-SMA in kidney of CRF rats,which could improve the condition of the CRF rats.Conclusion Erhuang compound granule is protective for chronic renal failure in kidney subtotal excision rats.
出处
《安徽医药》
CAS
2015年第10期1865-1868,共4页
Anhui Medical and Pharmaceutical Journal
关键词
复方二黄颗粒
大鼠
肾大部切除
慢性肾衰竭
Erhuang compound granule
rat
kidney subtotal excision
chronic renal failure
