外源性ghrelin在脓毒症小鼠肺脏炎症反应中的作用

Effect of exogenous ghrelin in lung inflammatory response of mice with sepsis

目的探讨生长素释放肽(ghrelin)在脓毒症小鼠肺脏炎症反应中的作用机制。方法 1通过腹腔注射内毒素(LPS)建立脓毒症模型,采用腹腔注射外源性ghrelin 200μg/kg进行干预。选取雌性昆明小鼠54只,随机分为对照组、模型组及ghrelin干预组,每组18只,对照组经腹腔注射等量生理盐水;模型组经腹腔内注射脂多糖6mg/kg;干预组先经腹腔注射入外源性ghrelin 200μg/kg,30min后再经腹腔注射脂多糖6mg/kg;2各组分别于3h、9h及18h随机处死6只小鼠,留取肺组织标本行HE染色,观察肺组织病理变化;ELISA法测定炎症因子TNF-α、IL-6的表达量;3统计分析使用SPSS 17.0软件,所得数据以x±s表示,用单因素方差分析来比较组之间总体差异,用SNK方法比较每两组的差异。结果与对照组相比,模型组及干预组中肺组织病理炎症反应加重,相关炎症因子TNF-α、IL-6的表达水平在各时间点均明显升高(P均<0.05),差异有统计学意义;与模型组相比,干预组中肺组织病理炎症反应减轻,相关炎症因子TNF-α、IL-6的表达水平在各时间点均明显降低(P均<0.05),差异有统计学意义。结论外源性ghrelin可通过降低早期炎症因子TNF-α、IL-6的表达,从而减轻脓毒症小鼠肺脏炎症反应,在脓毒症肺脏炎症反应中发挥保护作用。

Objective To discuss the regulating role of ghrelin in lung inflammatory response of mice with sepsis. Methods ①Set up sepsis model by injecting endotoxin into enterocoelia of mice, while injecting ghrelin （200μg/kg） into en-terocoelia of mice as intervention treatment , intraperitoneal injection equivalent amount of saline as a control was injected into enterocoelia of mice. 54 female Kunming mice were randomly divided into control group , model group and intervention group with ghrelin, each group contained 18 mice. Saline was injected into abdominal cavity of mice in control group. Model group with treated lipopolysaccharide 6mg/kg was injected into abdominal cavity. Intervention group： injected the ghrelin 200μg/kg into abdominal cavity, then injected lipopolysaccharide 6mg/kg into the abdominal cavity of mice after 30min. ②Six mice of each group were executed randomly respectively in 3h, 9h and 18h, collected specimen to corresponding test. Took each experi-mental group organization of the right upper lobe for HE staining , then observed the lung tissue pathology. Used enzyme linked immunosorbent （ELISA） to determinate the expression level of TNF alpha, IL-6.③The software of SPSS 17.0 was applicated for statistical analysis of data which was expressed with x±s. Comparison between groups was used by single factor analysis of vari-ance. Results Compared with the control group, lung tissue inflammation of model group and intervention group aggravated, the expression levels of TNF alpha, IL-6 at every time points were significantly higher （P〈0.05）, the difference had statistical significance. Compared with model group, lung tissue inflammation in the intervention group alleviated, the expression levels of TNF alpha, IL-6 at every time points were significantly lower （P〈0.05）, the difference had statistical significance. Conclusion Exogenous ghrelin can alleviate lung inflammatory response of mice with sepsis through decreasing the expression of TNF alpha and IL-6, it plays a protective role in sepsis lung inflammation.