黄芪联合应用阿托伐他汀对兔急性心肌梗死不同部位瞬间外向钾电流影响的研究

Effect of Huangqi Combined with Atorvastatin on Moment Outward Potassium Current of Different Parts in Rabbits with AMI

目的:研究黄芪联合应用阿托伐他汀对兔急性心肌梗死(AMI)后梗死边缘区及非梗死区心肌细胞瞬间外向钾电流(Ito)的影响。方法:建立AMI动物模型后,分别通过口服阿托伐他汀、腹腔注射黄芪注射液,酶解分离心室肌细胞,采用全细胞膜片钳记录技术记录梗死边缘区及非梗死区心外膜心肌细胞(Epicardium,Epi)Ito密度。结果:AMI梗死边缘区及非梗死区Epi细胞Ito密度与正常心肌细胞相比存在差异性;阿托伐他汀和黄芪分别使受抑制的梗死边缘区及非梗死区Ito密度恢复;阿托伐他汀和黄芪联合应用使梗死边缘区及非梗死区Ito密度进一步恢复。结论:AMI后梗死边缘区及非梗死区Epi细胞Ito密度存在电生理异常;阿托伐他汀和黄芪对心肌梗死梗死边缘区及非梗死区Ito密度的电生理异常有部分改善作用。

Objective：to study the effect of Huangqi combined with Atorvastatin on moment outward potassium current Ito on epi-cardium of infarction marginal zone and non-infarction zone in rabbits with AMI. Methods：AMI animal models prepared, Ito density of Epi was recorded with patch clamp technique by taking orally atorvastatin, getting intraperitoneal injection with Huangqi, separating ventricular myocardium through enzymolysis. Results： there was difference between Ito densities of Epi of infarction zone and normal Epi;Atorvastatin and Huangqi could respectively restore the Ito density of Epi of both zones;the combination could further restore the density. Conclusion：Ito density of Epi of post-AMI infarction marginal zone and none-infarction zone has electrophysiological abnormali-ty;Atorvastatin and Huangqi can improve the abnormality to a certain degree.