超高效液相色谱法测定参麦注射液中间产品中的人参皂苷Rg_1、Re和Rb_1

Determination of Rg_1,Re and Rb_1 in Intermediate Products of ShenMai Injection by UHPLC

采用超高效液相色谱法测定参麦注射液中间产品中的人参皂苷Rg1、Re和Rb1。参麦注射液中间产品经0.2μm滤膜过滤,以ACQUITY UPLC shield BEH RP18色谱柱为分离柱,以乙腈-水为流动相进行梯度洗脱,紫外检测波长为203nm。人参皂苷Rg1、Re和Rb1的质量浓度在0.081 8~0.409 2g·L^-1范围内与峰面积呈线性关系,检出限（3S/N）分别为0.652,0.479,0.916mg·L^-1。加标回收率在99.9%~100%之间,测定值的相对标准偏差（n=9）在0.47%~0.64%之间。

UHPLC was applied to the determination of ginsenosides（i.e.,Rg1,Re and Rb1）in the intermediate products of ShenMai injections.The sample of intermediates was filtrated through 0.2μm membrane filter.The filtrate was separated on ACQUITY UPLC shield BEH RP18 column with a mixture of acetonitrile and water as mobile phase in gradient elution.UV detection was made at the wavelength of 203 nm.Linear relationships between values of peak areas and mass concentrations of the Ginsenosides were kept in the same range of 0.081 8-0.409 2g·L^-1,with detection limits（3S/N）of 0.652,0.479,0.916 mg·L^-1.Values of recovery found by standard addition method were in the range of 99.9%-100%,with RSD′s（n=6）in the range of 0.47%-0.64%.