摘要
提出了一种测定氮丙啶的高效液相色谱分析方法。将自制氮丙啶单体与福林试剂（1,2-萘醌-4-磺酸钠盐）反应得到衍生物,通过NMR和IR表征验证衍生物结构。衍生物在Agilent ODB C18色谱柱（250mm×4.6mm,5μm）上分离,以乙腈-0.04mol·L^-1磷酸氢二钠混合溶液为流动相进行梯度洗脱,检测波长为258nm。氮丙啶的线性范围为1.00~20.0mg·L^-1,检出限（3S/N）为0.05mg·L^-1,测定值的相对标准偏差（n=6）小于2%。
A HPLC was applied to the determination of aziridine.Self-made aziridine monomer was derivatized with Folin′s reagent（1,2-naphthoquione-4-sulphonic acid）and the structure of the derivate was characterized by NMR and IR.For determination of aziridine,the derivate was seperated on Agilent ODB-C18（250mm×4.6mm,5μm）column,using a mixtrure of acetonitrile and 0.04mol·L^-1 sodium phosphate dibasic dodecahydrate solution as mobile phase in gradient elution.The wavelength used in UV-detection was 258 nm.The linearity range for aziridine was in the range of 1.00-20.0mg·L^-1,the detection limit（3S/N）was 0.05mg·L^-1,and RSD′s（n=6）found were less than 2%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2015年第9期1294-1297,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
浙江省公益性技术应用研究资助项目(2012C23012)
