RP-HPLC法测定人参花中11个人参皂苷含量

Determination of 11 kinds of ginsenosides from Ginseng Bud by RP-HPLC

目的：测定鲜人参花和人参花干品中11个人参皂苷的含量。方法：利用反相高效液相色谱法,使用Cosmosil 5 C18-MS（250 mm×4.6 mm,5μm）分析柱,流动相为乙腈-0.05 mol·L^-1磷酸二氢钾溶液,梯度洗脱,流速1 m L·min-1,柱温25℃,检测波长203 nm。结果：丙二酰基人参皂苷Rb1、Rb2、Rc、Rd及人参皂苷Rb1、Rc、Rb2、Rd、Re、Rg1、Rg2质量浓度分别在18.83~301.20μg·m L^-1（r=0.999 1）、19.01~304.20μg·m L^-1（r=0.999 8）、18.94~303.00μg·m L^-1（r=0.999 3）、18.79~300.60μg·m L^-1（r=0.999 4）、18.56~297.00μg·m L^-1（r=0.999 0）、18.86~301.80μg·m L^-1（r=0.999 1）、18.94~303.00μg·m L^-1（r=0.999 5）、37.58~601.20μg·m L^-1（r=0.999 6）、37.58~601.20μg·m L^-1（r=0.999 1）、19.05~304.80μg·m L^-1（r=0.999 3）、18.68~298.80μg·m L^-1（r=0.999 6）范围内与峰面积呈良好的线性关系,平均加样回收率（n=6）在98.3%~102.9%之间,RSD≤3.0%。鲜人参花中11个人参皂苷平均含量为10.80%,其中丙二酰基人参皂苷的平均含量为3.46%;人参花干品中11个人参皂苷含量为10.68%,其中丙二酰基人参皂苷的平均含量为1.21%。结论：本实验方法简单,可高效地测定鲜人参花和人参花干品中11个人参皂苷的含量。

Objective： To establish a method for the determination of 11 kinds of ginsenosides from both fresh and dried Ginseng Bud. Methods： RP- HPLC was conducted on a Cosmosil 5 C18- MS column（ 250 mm × 4. 6 mm,5μm） with the mobile phase of acetonitrile- 0. 05 mol·L^-1potassium dihydrogen phosphate solution by gradient elution at a flow rate of 1 m L·min- 1. The column temperature was maintained at 25 ℃,and the detection wave length was 203 nm. Results： The linear response ranged from 18. 83-301. 20 μg·m L^-1for malonyl ginsenoside Rb1（ r =0. 999 1）,19. 01-304. 20 μg·m L^-1for malonyl ginsenoside Rb2（ r = 0. 999 8）,18. 94-303. 00 μg·m L^-1for malonyl ginsenoside Rc（ r = 0. 999 3）,18. 79-300. 60 μg·m L^-1for malonyl ginsenoside Rd（ r = 0. 999 4）,18. 56-297. 00 μg·m L^-1for ginsenoside Rb1（ r = 0. 999 0）,18. 86-301. 80 μg·m L^-1for ginsenoside Rc（ r = 0. 999 1）,18. 94-303. 00 μg·m L^-1for ginsenoside Rb2（ r = 0. 999 5）,37. 58-601. 20 μg·m L^-1for ginsenoside Rd（ r =0. 999 6）,37. 58-601. 20 μg·m L^-1for ginsenoside Re（ r = 0. 999 1）,19. 05-304. 80 μg·m L^-1for ginsenoside Rg1（ r = 0. 999 3） and 18. 68-298. 80 μg·m L^-1for ginsenoside Rg2（ r = 0. 999 6）. The average recoveries（ n = 6）were between 98. 3%-102. 9%,and the RSD ≤3. 0%. In fresh Ginseng Bud,the contents of 11 kinds of ginsenosides was 10. 80%,and the content of malonyl ginsenosides was 3. 46%; in dried Ginseng Bud,the contents of 11 kinds of ginsenosides was 10. 68%,and the content of the malonyl ginsenosides was 1. 21%. Conclusion： The established method is simple,fast and accurate,and can be applied for the determination of the content of 11 kinds of ginsenosides from both fresh and dried Ginseng Bud.