杭州郊区马铃薯A病毒分离物的基因组3’-末端序列测定及系统进化分析

Determination and phylogenetic tree analysis of 3'-part genomic sequence of a potato virus A isolates occurred in Hangzhou

采用马铃薯Y病毒属通用引物Sprimer扩增了引起浙江杭州郊区发生的马铃薯病毒基因组 3’ 末端 16 87核苷酸序列 ,它包括NIb ,CP和 3’ UTR ,3’ 末端含Poly(A)尾 ,蚜传基序DAG位于多聚蛋白第 2 15～ 2 17氨基酸。BLAST分析表明该病毒为马铃薯A病毒 (PVA) ,它与已报道的 19个PVA分离物CP氨基酸进行比较 ,PVA浙江分离物与荷兰Alc分离物同源性最高 ,为 99.6 %。系统进化树分析表明 ,PVA分离物间存在一些明显的进化群体。

Using a universal primer of family Potyviridae, potato virus A (PVA) was identified from potatoes in Hangzhou city. One thousand six hundred and eighty seven nucleotide sequence of 3' terminal genome was determined, including NIb, CP and 3' UTR. DAG motif, which was closely related to the aphid transmission, was identified at the position 215 217aa in polyprotein. Comparison to other 19 isolates of PVA suggested that Hangzhou isolate was most similar to Alc isolate from the Netherlands, and their homology of amino acid sequence of CP was 99.6%. Phylogenetic tree analysis confirmed that some significant groups of PVA isolates existed.