实时荧光定量RT-PCR检测STAT3 mRNA在食管鳞状细胞癌中的表达

EXPRESSION OF STAT3 MRNA IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA BY REAL TIME RT-PCR

目的采用实时荧光定量RT-PCR检测STAT3mRNA在食管鳞状细胞癌癌组织及癌旁正常组织中的表达水平,研究食管鳞状细胞癌STAT3mRNA与临床病理特征的关系。方法对100例食管鳞状细胞癌患者术后肿瘤组织及癌旁正常食管粘膜组织采用实时荧光定量RT-PCR方法检测STAT3 mRNA表达,分析其在二者中的表达及其与临床病理特征的关系。结果STAT3mRNA在癌组织中的表达(0.0027±0.0064)是癌旁正常组织(0.0014±.0031)的2倍;存在显著性差异,(P<0.05)。STAT3mRNA表达与淋巴结转移(t=2.970 P<0.05)和分化程度(t=5.936 P<0.05)相关,与临床分期、浸润深度、肿瘤大小无明显相关(P>0.05)。结论实时荧光定量RT-PCR技术可准确可靠地反映各靶基因的表达倍数;在食管鳞状细胞癌组织中STAT3mRNA的表达高于癌旁正常组织,是正常组织的2倍,证实STAT3在食管鳞状细胞癌的发生发展中发挥着重要作用,STAT3mRNA的表达与淋巴结转移和分化程度相关。

Objective The aim of this study was to detect the expression of STAT3 mRNA in esophageal squamous cell carcinoma（ESCC） by real time RT-PCR and to investigate STAT3 mRNA expression and the correlation with clinicopathological parameters. Methods Tumor specimens were collected from 100 pa- tients with ESCC who underwent complete resection . We investigate the STAT3 mRNA expression levels in ESCC by real time RT-PCR,the expressions of ERCC1 were observed and correlated with clinicopatho- logical characteristics. Results In the cancer specimens the STAT3 mRNA was（0. 0027 ±0. 0064）, in the control specimens was（0. 0014± 0031）. Between the cancer tissue and control group,the statistical differ- ence was significant（ P 〈 0.05）. STAT3 mRNA expression was associated with lymph node metastasis（t =2. 970 P 〈 0.05） and cell differentiation （t =5. 936 P 〈 0.05） but not associated with tumor size,depth of invasion, clinical stage cell differentiation （ P 〉0.05）. Conclusion The Real-time PCR was the simple,accuracy and reliable method to report the ratio of the target gene in the cancer tissue to the normal group;In the ESCC carcinoma tissue, the expression of STAT3 mRNA were statistically high than that of normal tis- sue, the increased fold was 2. The over-expression of STAT3 mRNA was one of the important phenotypes and characteristics in ESCC carcinomatous change and associated with lymph node metastasis and cell differentiation in ESCC .