蒸发光散射高效液相色谱法测定黄芪饮片中的黄芪甲苷含量

Determination of Astragaloside Ⅳ in Astragalus Decoction Pieces by HPLC–ELSD

采用蒸发光散射高效液相色谱法测定黄芪饮片中黄芪甲苷的含量。黄芪饮片分别用甲醇和正丁醇提取，以NaOH溶液洗涤，蒸干，再用甲醇溶解，采用Agilent SB—C18柱（250mm×4．6mill，5μm）分离测定，流动相为乙腈-水（32：68），流量为1．0mL／min，漂移管温度为80℃。方法检出限为1．5μg／mL，黄芪甲苷溶液的质量浓度在2—12μg／mL范围内与色谱峰面积线性关系良好（r^2=0．9996），测定结果的相对标准偏差为0．65％（n=6），平均加标回收率为99．6％。该方法稳定可靠，可用于黄芪药材及其生物制品中黄芪甲苷含量的测定。

HPLC-ELSD method was used to determine astragaloside Ⅳ in astraglus decoction pieces. The sample of astragalous decoction pieces was extracted by methanol and n-butyl alchol, after cleaning with NaOH solution, the sample was dried and disolved in methanol, and was determined by Agilent SB-C18 column （250 mm × 4.6 mm, 5μm）, acetonitrile-water（32 ： 68） was as the mobile with the flow rate of 1.0 mL/min, the drifttube temperature was 80 ℃ . The detection limit of the method was 1.5 μ g / mL. The linear range was 2-12μ g / mL（r^2=0.999 6）. The RSD of determination results was 0.65%（n=6）. The average recovery of astragaloside Ⅳ was 99.6%. This method is accurate, reliable and with good reproducibility, and it can be used for the determination of astragaloside Ⅳ in Radix Astragali and its products.