氯诺昔康联合地佐辛超前镇痛对切口痛大鼠白细胞介素-6及脊髓环氧化酶-2表达的影响

Effect of lornoxicam combined with dezocine preemptive analgesia on the expressions of serum interleukin-6 and cyclooxygenase-2 in spinal cord in rats with incision pain

目的:观察氯诺昔康联合地佐辛对切口痛大鼠模型的超前镇痛效应,探讨其可能作用机制。方法:40只Wistar大鼠随机分为空白对照组、切口痛模型组、氯诺昔康组、地佐辛组和氯诺昔康联合地佐辛组(联合用药组)各8只。空白对照组不做任何处理;切口痛模型组术前30 min尾静脉注射0.9%氯化钠注射液;氯诺昔康组术前30 min尾静脉注射氯诺昔康2 mg/kg;地佐辛组术前30 min尾静脉注射地佐辛5 mg;联合用药组术前30 min尾静脉注射氯诺昔康1 mg/kg,地佐辛2.5 mg。术后采用累计疼痛评分法给予切口痛大鼠疼痛评分,术后3 h大鼠尾静脉采血,于脊髓膨大处取脊髓,分别检测血清白细胞介素(IL)-6和脊髓环氧化酶(COX)-2的含量。结果:切口痛模型组大鼠累计疼痛评分均明显高于氯诺昔康组、地佐辛组和联合用药组(P<0.01),而联合用药组大鼠累计疼痛评分均较单用药组显著下降(P<0.01);联合用药组大鼠血清IL-6和脊髓COX-2的表达均显著低于单用药组(P<0.01)。结论:氯诺昔康联合地佐辛能通过抑制切口痛大鼠血清IL-6和脊髓COX-2的表达,降低大鼠累计疼痛评分,表现出显著的超前镇痛作用。

Objective：To observe the preemptive analgesia effects of lornoxicam combined with dezocine on the rat with incision pain, and investigate its possible mechanism. Methods： Forty Wistar rats were randomly divided into the control group （ without any treatment） ,incision pain model group（ treatment with preoperative 30 min tail vein injection of saline）, lornoxicam group （treatment with preoperative 30 min tail vein injection of 2 mg/kg of lornoxicam）, dezocine group （treatment with preoperative 30min tail vein injection of 5 mg of lornoxicam） and lornoxicam combined with dezocine group（ treatment with preoperative 30 min tail vein injection of 1 mg/kg of lornoxicam combined with 2.5 mg of Iornoxicam）. The pain score of rats with incision pain after operation was investigated using the cumulative pain score method. The interleukin （IL）-6 expression in the rat tail vein and cyclooxygenase （COX）-2 level in spinal cord were measured after 3h of operation. Results：The cumulative pain score in incision pain model group was significantly higher than that in lornoxicam group, dezocine group and lornoxicam combined with dezocine group （ P 〈 0. 01 ）. The cumulative pain score in lornoxieam combined with dezocine group was significantly lower than that in single drug group（P 〈 0.01 ）. The expressions of serum IL-6 and COX-2 in spinal cord in lornoxicam combined with dezocine group were significantly lower than that in single drug group （ P 〈 0.01 ）. Conclusions： Lornoxicam combined with dezocine can inhibit the expressions of serum IL-6 and COX-2 in spinal cord and decrease the pain score in rat with incision pain, which plays the preemptive analgesia effects.