子痫前期患者胎盘中miRNA-21及Th17相关因子的表达及其关系被引量：2

Expression of miRNA-21 and correlation factors associated with Th17 cells and analysis their relations in placenta of patients with preeclampsia

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摘要目的探讨胎盘中Th17细胞相关因子IL-17、IL-21、RORc、人类白细胞抗原-G（HLA-G）蛋白及胎盘中微小RNA（micro-ribonucleic acid,miRNA）-21在子痫前期发病中的作用。方法选择孕33~37周行剖宫产分娩的子痫前期产妇20例作为子痫前期组,选择孕38周以上自愿要求行剖宫产产妇20例作为正常对照组。采用实时荧光定量-聚合酶链反应技术检测胎盘组织中IL-17、IL-21、RORc、HLA-G蛋白以及miRNA-21的表达量。结果子痫前期组胎盘中IL-17、IL-21及miRNA-21相对表达量均明显高于正常对照组（P均〈0.01）,RORc和HLA-G相对表达量明显低于对照组（P均〈0.05）。2组胎盘组织中IL-17、IL-21及miRNA-21表达均呈正相关,RORc和HLA-G表达均呈负相关。结论子痫前期患者胎盘组织中miRNA-21表达升高,促使Th17细胞激活,导致IL-17和IL-21分泌增加;同时miRNA-21表达升高,使得RORc mRNA和HLA-G mRNA的稳定性降低,产生基因沉默影响转录,导致RORc和HLA-G表达减少。IL-17、IL-21、RORc、HLA-G蛋白以及miRNA-21可能与子痫前期发病有关。 Objective It is to study the predictive value of IL - 17, IL - 21, RORc, HLA - G associated with Th17 cells and micro-ribonucleic acid - 21 （ miRNA - 21 ） in placenta on the pathogenesis of preeclampsia. Methods 20 pregnant women with preeclampsia with 33 -37weeks＇ gestation undergoing cesarean section were selected as preeclampsia group, 20 healthy pregnant women with more than 38 weeks＇ gestation volunteered for cesarean section as normal control group. Expression of IL - 17, IL -21, RORc , HLA - G and miRNA -21 in placenta were measured by Polymerase Chain Reaction （PCR）. Results The relative expressions of IL - 17 and IL - 21 in placenta were higher, while that of RORc and HLA - G were lower in preeclamp- sia group than those in normal control group （P 〈 0.01 or 〈 0.05 ）. The relative expressions of IL- 17, IL- 21 and miRNA - 21 in placenta in both groups were positively correlated,, the relative expressions of RORc and HLA - G in placenta were nega- tively correlated. Conclusion We found that the relative expressions of miRNA -21in placenta was more higher in preeelamp- sia group. Higher expression of miRNA - 21 may make Th17 cells activated, then increased IL - 17 and IL - 21 secretion. At the same time ,higher expression of miRNA -21 may influence the RORc mRNA and HLA -G mRNA stability, then reduced the expression of RORc and HLA - G.

作者朱丽朱华英徐玲娣陈廷美刘颂平

机构地区江苏大学医学院江苏大学附属第四人民医院镇江市妇幼保健院

出处《现代中西医结合杂志》 CAS 2015年第29期3193-3195,3198,共4页 Modern Journal of Integrated Traditional Chinese and Western Medicine

基金江苏省卫生厅重大项目(H201249)

关键词子痫前期 MIRNA-21 Th17细胞相关因子 preeclampsia miRNA -21 correlation factors associated with Th17 cells

分类号 R972.4 [医药卫生—药品]

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