不同来源的IL-10对脑疟小鼠免疫病理损伤的调控作用

Effect of different sources of IL-10 on regulating immune pathology in mice infected with cerebral malaria

目的探讨不同来源的IL-10对伯氏疟原虫感染过程中免疫病理损伤的调控作用。方法用伯氏疟原虫ANKA感染C57BL/6小鼠,Giemsa染色计数红细胞感染率;感染前和感染后3、5、8天制备脾细胞悬液,流式细胞术检测脾细胞悬液中分泌IL-10的Tregs和Bregs百分比;ELISA方法检测脾细胞培养上清IFN-γ和IL-10水平。结果 C57BL/6小鼠感染后7至11天死于脑型疟疾(CM),分泌IL-10的Tregs和Bregs百分比于感染后5天和8天分别达峰值。C57BL/6小鼠脾细胞培养上清中IFN-γ和IL-10水平于感染后出现升高,感染后第5天达峰值,随后均出现下降,但仍高于正常对照组,P<0.05。结论脑型疟疾发生过程中,以IL-10为核心的免疫调节网络能够调控病理损伤的程度。不同来源的IL-10在疟疾发生、发展不同阶段发挥着不同的调节作用。

Objective To explore the effect of different sources of IL-10 on regulating the immune pathology in mice infected with cerebral malaria. Methods C57BL/6 mice was infected by intraperitoneal injection of 1 × 106 Plasmodium berghei （P. b） ANKA parasited erythrocytes. Parasitemia of individual mice was monitored by the microscopic examination of blood smear stained with Giemsa. Spleen cell suspension was prepared from the mice sacrificed on days 0, 3, 5, 8 post infection. The percentages of Tregs and Bregs were analyzed by flow cytometry. The levels of IFN-γ and IL-10 in spleen supernants were measured by ELISA. Results C57BL/6 mice died between 7 and 11 days after infection with cerebral malaria （CM）. The percentages of IL-10-secreting Tregs and IL-10-secreting Bregs reached a peak after infection on day 5 post infection and day 8 post infection respectively. The levels of IFN-γ and IL-10 in cul- tured splenocyte supernatants of C57BL/6 mice began to increase after infection with peaks on day 5 post infection and subsequently decreased, but still higher than normal control group. ological injury play different IL-10 as the core of the immune regulatory network can regulate the level of pathduring the process of cerebral malaria occurrence. ＂The different sources of IL-10 roles in different stage of malaria infection.