摘要
目的应用微小核糖核酸(microRNA)芯片技术初步筛选Ⅳ、Ⅳ+Ⅴ型狼疮性肾炎患者血清microRNA,探讨血清microRNA在狼疮性肾炎病理分型中的作用。方法从北京大学深圳医院肾内科生物标本库中选取Ⅳ、Ⅳ+Ⅴ型狼疮性肾炎血清各4例,作为试验组;同时选取4例健康人血清作为对照组,使用microRNA芯片技术筛选差异表达的血清microRNA。结果Ⅳ型和Ⅳ+Ⅴ型共有部分microRNA,其中22个有显著差异,Ⅳ型特异性microRNA有29个,Ⅳ+Ⅴ型特异性microRNA有3个。结论血清microRNA具有作为鉴别Ⅳ、Ⅳ+Ⅴ型狼疮性肾炎生物标志物的潜能。
Objective To preliminary screen serum microRNA which was related to classⅣand classⅣ＋Ⅴlupus nephritis（LN）by using microRNA array technology,and explore the role of serum microRNA in renal pathological classification of lupus nephritis.Methods Eight serum samples of lupus nephritis patients from biological specimen library in our hospital were enrolled.Experimental group was composed of 4classⅣ cases and 4classⅣ＋Ⅴ cases,control group was composed of 4healthy persons.MicroRNA array was used to screen the differentially expressing microRNA.Results There were part of same miRNA in classⅣand classⅣ＋Ⅴ LN,among which expression of 22 miRNA were significantly different,29 miRNA of classⅣ was unique,3miRNA of classⅣ＋Ⅴ was unique.Conclusion Serum miRNA has a potential to serve as a biomarker to identify classⅣ and classⅣ＋Ⅴlupus nephritis.
出处
《重庆医学》
CAS
北大核心
2015年第27期3745-3747,3751,共4页
Chongqing medicine
基金
广东省自然科学基金博士启动项目资助(S2013040015040)
