E-cad、Ep-CAM在头颈部腺样囊性癌中表达的临床意义

Expression of E-cad and Ep-CAM in head and neck adenoid cystic carcinoma and its clinical significance

目的研究细胞黏附分子(CAM)上皮性钙黏连蛋白(E-cad)、上皮细胞黏附分子(Ep-CAM)在头颈部腺样囊性癌(ACC)中的表达情况,探讨其在头颈部ACC发生、发展及侵袭转移中的作用。方法应用免疫组织化学EnVision法检测60例头颈部ACC及30例正常腺体组织中E-cad、Ep-CAM的表达情况,采用Image-Pro Plus 6.0图像分析系统对免疫组织化学结果进行分析。结果 E-cad在头颈部ACC中的表达低于正常腺体组织(P<0.05),Ep-CAM在头颈部ACC中的表达高于正常腺体组织(P<0.05);E-cad在头颈部ACC组织中的相对低表达分别与ACC的病例分型、临床分期、有无神经症状及有无复发转移有关(P<0.05);Ep-CAM在头颈部ACC组织中的高表达分别与ACC的病例分型、临床分期、有无神经症状及有无复发转移有关(P<0.05)。E-cad与Ep-CAM在头颈部ACC中的表达呈负相关关系(r=-0.890,P=0.000)。结论 E-cad可能具有抑制头颈部ACC发生、发展的作用,Ep-CAM在头颈部ACC中表达越高,提示头颈部ACC恶性程度越高。

Objective To study the expression of E-cad,Ep-CAM in head and neck ACC and clarify its role and in the malignant progress,invasion and metastasis of ACC.Methods The expression of E-cad and Ep-CAM in 60 cases of head and neck ACC specimens and 30 cases of normal gland tissues were detected by immunohistochemistry EnVision method.The result of immunohistochemistry was conducted by the Image-Pro Plus 6.0image analysis system.Results The expression of E-cad in head and neck ACC was statistically significant lower than that in normal gland tissue（P〈0.05）.The expression of Ep-CAM in head and neck ACC was statistically significant higher than that in normal gland tissue（P〈0.05）.The decreasing expression of E-cad in head and neck ACC was closely related to the different pathological patterns,clinical stages,neurological symptom and local recurrences or distant metastases（P〈0.05）.The high expression of Ep-CAM in head and neck ACC was closely related to the different pathological patterns,clinical stages,neurological symptoms and local recurrences or distant metastases（P〈0.05）.There was a negative correlation about the expression between E-cad and Ep-CAM in head and neck ACC（r=-0.890,P=0.000）.Conclusion E-cad may act as a inhibitor in the occurrence and development of ACC.The higher expression of Ep-CAM in head and neck ACC showed that ACC had a more poorer prognosis.