林甸鸡绿壳蛋、隐性白、快慢羽性状的遗传基础分析

Genetic Analyses on Blue Eggshell, Recessive White and Feathering Rate Traits in Lindian Chicken Population

试验旨在研究影响绿壳蛋、隐性白和快慢羽性状的SLCO1B3、TYR和K基因型在林甸鸡群中的分布情况。通过PCR和电泳技术分别对影响林甸鸡绿壳蛋、隐性白和快慢羽性状的SLCO1B3、TYR和K基因突变位点进行分型工作,发现产绿壳蛋的林甸鸡携带SLCO1B3-O等位基因,SLCO1B3-OO型占12.5%,SLCO1B3-Oo型占87.5%;林甸鸡中白羽个体基因型为TYR-cc,非白羽个体中有19.3%的鸡携带TYR-c等位基因,基因型为TYR-Cc;在林甸鸡中检测到慢羽型鸡占7.8%,快羽型鸡占92.2%。通过追溯试验群体的系谱结构,发现影响林甸鸡绿壳蛋性状SLCO1B3基因、隐性白性状的TYR基因和快慢羽性状的K基因的突变位点在上下代传递过程中符合孟德尔遗传规律。

The blue eggshell, recessive white and feathering rate traits are genetically controlled by three differentgenes of SLCO1B3,TYR and K, respectively. The Lindian chicken is a local breed in Lindian county,Heilongjiang province （northeastern part of China）. The aim of this study was to evaluate the genotype distribution of these three genes in Lindian chicken population, respectively. The genotypes of SLCO1B3, TYR and K genes were checked directly by PCR and electrophoresis method. We found chickens producing blue eggshell carried O allele of SLCO1B3 gene, where genotypes OO and Oo accounted for 12.5% and 87.5% ,respectively. Genotypes of individuals with white feather were recessive type TYR-Cc. Carriers of allele c of TYR gene had TYR-Cc genotype,accounting for 19.3% of Lindian chickens with non-white feather. The birds of the slow- and fast-feathering types accounted for 7.8% and 92.2%,respectively. By tracing the pedigree relationship between all experimental birds, we found that the alleles of SLCO1B3, TYR and K genes were transmitted conforming to the law of Mendelian inheritance.