飞行时间质谱技术对慢性病患者焦虑相关蛋白质水平的判断价值

Judgment Value of SELDI on Related Anxiety Protein Level for Patients with Chronic Diseases

目的探讨慢性病患者焦虑情绪的发生情况,以及在焦虑状态下,其血清焦虑蛋白质指纹图谱的变化规律。方法采用便利抽样法,选取慢性病患者和正常人设为观察、对照组各30例。应用焦虑自评量表、表面增强激光解吸离子化飞行时间质谱技术(surface-enhanced laser desorption/ionization time-of-flight mass spectrometer,SELDI-TOF-MS),对2组进行评估和检测;根据焦虑得分和SELDI检测结果,筛选出焦虑情绪症状的样本,对心理问题进行4周心理干预后,再评估焦虑及检测SELDI;应用SPSS 16.0和Biomarker Wizard 3.1,对观察组心理干预前后、2组干预后的焦虑评分结果、焦虑蛋白质指纹图谱进行比较分析。结果观察组、对照组的焦虑得分差异有统计学意义(t=6.43,P<0.01);通过SELDI检测得知,观察组焦虑蛋白阳性15例,对照组焦虑蛋白阳性4例,阳性率差异有统计学意义(χ2=9.32,P<0.01);经心理干预后,观察组焦虑评分比干预前显著降低(t=7.71,P<0.01);且该组焦虑蛋白质指纹图谱无阳性病例出现,与心理干预前阳性率差异有统计学意义(P<0.01)。结论慢性病患者焦虑情绪的发生率相对较高,在用SELDI技术获取的蛋白质指纹图谱上也会有焦虑蛋白的存在,SELDI技术也可以作为筛查慢性病患者是否存在焦虑情绪的一项客观筛查方法。

Objective To explore the incidence of anxiety of patients with chronic disease and the variation of the serum proteins fingerprints spectrum when patients with anxiety. Methods With random sampling, 30 cases of patients with chronic diseases and another 30 healthy persons were divided into observation group and control group. All the research object were assessed by self-rating anxiety scale （SAS） and their sera were detected utilizing SELDI-TOF-MS （surface-enhanced laser desorption / ionization time-of-flight mass spectrometer）. According to the results of SAS and SELDI test, we conducted psychological intervention for the existing mental health problems for four weeks, then tested SAS and SELDI for another time. The variation of SAS scores and SELDI fingerprints spectrum on F-B psychological intervention were observed and compared. 16.0 SPSS and Biomarker Wizard 3.1 were applied to analyze the result of SAS and protein fingerprints spectrum on two groups respectively. Results Anxiety scores in both groups indicated statistical significance （t=6.43,P〈0.01） and SELDI detection showed that there were 15 positive cases in observation group and 4 in control group, which was statistically significant by χ2test （P〈0.01）. After the psychological intervention, the SAS graded down to 33.73 ±7.887 and no case with positive was found in observation group. The rate of patients with positive anxiety protein fingerprints spectrum before and after psychological intervention revealed statistical significance （ P〈0.01）.Conclusion There is relatively high incidence of anxiety among patients with chronic disease. We can find the related anxiety protein in the protein fingerprint acquisition using SELDI technology and the SELDI can also be used as a objective screening method for screening the existence of anxiety among patients with chronic diseases.