龈沟液OPG/RANKL表达与慢性牙周炎患者龈沟出血指数的相关性

The correlation of OPG/RANKL expression level of gingival crevicular fluid to sulcus bleeding index in patients with chronic periodontitis

目的分析慢性牙周炎患者龈沟液护骨素(osteoprotegerin,OPG)和核因子κB受体活化因子配体(receptor activator of nuclear factor-KB ligand,RANKL)表达与其龈沟出血指数的关系。方法以吸潮纸尖法,取37例慢性牙周炎患者(观察组)和同期入院体检的31例健康志愿者(对照组)的龈沟液。采用免疫荧光试验分析OPG和RANKL的表达强度;并以简单线性回归法测试OPG/RANKL表达与龈沟出血指数的相关性。结果与对照组比较,观察组患者OPG和OPG/RANKL表达强度明显偏低,RANKL表达强度明显偏高,且各指标的组间差异均有统计学意义(P<0.001)。经线性回归分析,观察组患者的OPG/RANKL的表达情况与其出血指数呈显著负相关(y=-3.7755x+3.6212,R2=0.6651,P<0.05)。结论 OPG/RANKL/RANK系统参与了慢性牙周炎的发生和发展,可作为衡量其炎症性出血程度的客观指标。

Objective To examine the relationship between the level of osteoprotegerin （ OPG） and receptor activator of nuclear factor-KB ligand （ RANKL） expression and sulcus bleeding index.Method The gingival crevicular fluid of 37 patients with chronic periodontitis （ chosen as the experimental group ） and 31 healthy volunteers were abstracted with moisture absorption paper point method.Their OPG and RANKL expression level were detected with immunofluorescence test and the correlation of OPG/RANKL expression and sulcus bleeding index were analyzed with simple linear regression. Findings Compared with the control group, the OPG and OPG/RANKL expression level were lower significantly and the RANKL expression level were higher significantly （P〈0.001）.Negative correlation was found between the OPG/RANKL expression level and sulcus bleeding index （y=-3.7755x ＋3.6212,R2 =0.6651,P〈0.05）.Conclusion OPG/RANKL/RANK system is involved in the occurrence and development of chronic periodontitis.It can be used as an objective index to measure the degree of inflammatory bleeding.