落叶松愈伤组织诱导体系优化

The Callus Induction Medium Optimization of Larch

以落叶松成熟合子胚为作为外植体材料,对其按照不同处理方式进行消毒、且以不同种类培养基和激素种类及浓度进行愈伤组织的诱导,测算愈伤组织的体积。统计分析结果表明:将去皮种子用流水冲洗2 d,用70%酒精消毒1min转入4%Na Cl O消毒10 min后,再将剥出的胚放入0.5%Na Cl O消毒2 min胚存活率最高;在光照条件下,MS培养基为愈伤组织诱导的优良培养基,2.0 mg·L-12,4-D+1.0 mg·L-16-BA或1.0 mg·L-1NAA+0.5mg·L-16-BA激素组合为最佳。

The Larch mature zygotic embryos were taken as the explants. They were disinfected according to different treatment methods, and induced by use of different medium and hormone types and concentration, then the callus volume were calculated. Statistical analysis results showed that the peeled seeds were washed with water for 24 hours - 48 hours, disinfected in 70% alcohol for 1 minute and disinfected into 4% NaClO for 10 minutes, and then the embryo was stripped out in the 0.5% NaClO disinfection for 2 minutes and the suvival rate of embryos was the highest ; In the light conditions, MS medium was good medium for callus induction. It could be seen that 2. 0 mg ·L^-12,4-D ＋ 1.0 mg ·L^-1 6-BA or 1.0 mg·L^-1 NAA ＋ 0. 5 mg·L^-1 6-BA were the best hormone combination.