一氧化氮在打破杜梨种子休眠中的作用

Effect of Nitric Oxide on Breaking Pyrus betulifolia Seed Dormancy

处于休眠状态的杜梨种子,经不同浓度硝普钠(SNP),2-苯基-4,4,5,5-四甲基咪唑啉-1-氧-3-氧化物(PTIO),钨酸钠(Na2WO4),zhi NG-氮-L-精氨酸-甲酯(L-NAME)浸泡处理24 h,在培养过程中统计杜梨种子的发芽率,以研究一氧化氮(NO)在杜梨种子休眠中的作用,以及杜梨种子解除休眠过程中NO的合成途径。结果表明:NO可以有效的打破杜梨种子休眠,其中10 m M SNP效果最好,经10 m M SNP处理后杜梨种子的萌发率达到56.7%,显著高于对照(20.0%);在打破杜梨种子休眠中,一氧化氮合酶(NOS)途径参与NO合成,而硝酸还原酶(NR)途径没有参与,经L-NAME处理杜梨种子萌发率为15.6%,显著低于对照,而经Na2WO处理对杜梨休眠影响没有显著影响。

Pyrus betulifolia dormant seeds were soaked and treated for 24 hours with the different concentrations of Sodium nitroprusside （ SNP ） , 2-phenyl-4, 4, 5, 5-tetramethylimidazo-line-l-oxyl-3-oxide （PTIO） ,Sodium tungstate（ Na2WO4 ）and ZhiN^Gn-L-arginine methyl ester （L-NAME） ,and the percentage germination was calculated in the training process, aiming to find effects of Nitric oxide （NO） and the synthesis pathway of nitric oxide on seed dormancy of P. betulifolia. The results were as follows ： NO could break the seed dormancy for germination of Pyrus betulifolia, 10 mmol · L^-1 of SNP treatment was better in promoting germination, the germination rate of seeds pretreated with 10 mM SNP reached 56. 7%, significantly higher than the control（20.0% ） ;In breaking the dormancy of Pyrus betulifolia seeds, NO came from nitric oxide synthase（NOS） but not Nitrate reductase （NR） pathway, the germination rate of seeds pretreated with L-NAME was 15.6%, significantly higher than the control, but Na2WO4 Pretreatment didn＇t have any effect.