姜黄素促进紫杉醇抑制肿瘤细胞的增殖

Curcumin Promotes the Inhibiting Role of Paclitaxel in Cancer Cells Proliferation

培养的人喉癌细胞Hep-2及肝癌细胞HepG-2,分别用不同浓度姜黄素（CUR,0~50μmol/L）和紫杉醇（PTX,0~5nmol/L）单独处理或联合处理（5μmol/L CUR＋0.5nmol/L PTX、10μmol/L CUR＋0.5nmol/L PTX）.处理48h后,用WST-1细胞增殖试剂盒检测细胞增殖情况,再用结晶紫染色检测活细胞的密度,最后用Hoechst 33258凋亡染色试剂盒检测细胞凋亡情况.结果显示：姜黄素或紫杉醇单独处理对Hep-2细胞和HepG-2细胞的增殖均有抑制作用,并呈现出一定剂量效应关系;姜黄素与紫杉醇联合处理后细胞的增殖速率要比紫杉醇单独处理的明显降低;同时,姜黄素与紫杉醇联合处理后细胞的凋亡要比紫杉醇单独处理的更为明显.上述研究结果说明,姜黄素对紫杉醇抑制肿瘤细胞Hep-2和HepG-2的增殖有促进作用,并能促进紫杉醇诱导细胞的凋亡.

The human laryngeal carcinoma Hep-2 and hepatocarcinoma HepG-2 cells are cultured with different concentrations of curcumin（CUR,0~50μmol/L）and paclitaxel（PTX,0~5nmol/L）separately or in combined treatment with 5μmol/L CUR＋0.5nmol/L PTX and 10μmol/L CUR＋0.5nmol/L PTX for 48 h.After that,cell proliferation,the density of living cells and cell apoptosis are measured by WST-1assay,crystal-violet staining and Hoechst 33258 staining,respectively.The results show that both curcumin and paclitaxel can inhibit Hep-2and HepG-2cell proliferation in dosedependent manner.The proliferation rate of cells combined-treated with curcumin and paclitaxel is obviously lower than that of the cells treated with paclitaxel alone.Besides,the apoptosis in the group of combined treatment with curcumin and paclitaxel is much obvious than that of the paclitaxel-treated group.Thus,all results illustrate curcumin can promote paclitaxel inhibiting cell proliferation as well as inducing apoptosis in Hep-2and HepG-2cells.