摘要
建立了测定N-苯基哌嗪盐酸盐的方法。采用反相高效液相色谱法,色谱柱采用十八烷基硅烷键合硅胶柱,流动相为磷酸二氢钾缓冲液(0.05mol/L,pH=3.5)∶乙腈=50∶50,检测波长为249nm,流速为0.8mL/min。苯基哌嗪盐酸盐回收率为99.4%~102.0%(n=9),重复性试验的相对标准偏差小于2.0%(n=6),有关物质与主峰有较好的分离度。此法简单、重现性好、结果准确可靠,适用于N-苯基哌嗪盐酸盐的质量控制。
The detection method for N-phenyl piperazine hydrochloride was established.Reverse phase HPLC with eighteen alkyl silane bonded silica gel as the HPLC column filler was used.Sodium dihydrogen phosphate/acetonitrilemobile(V/V=50/50)was the mobile phase;249nm was the detection wavelength;and the flow velocity,was 0.8mL/min.The rate recovery of phenyl piperazine hydrochloride was 99.4%~102.0%(n=9);the standard deviation of the determination was less than 2.0%(n=6);and the degree of separation was relatively high.The method was simple,accurate,reliable and reproducible.It could be applied to quality control of production N-phenylpiperazine hydrochloride in industry.
出处
《化学世界》
CAS
CSCD
2015年第9期529-533,共5页
Chemical World
基金
国家重点基础研究发展计划(973计划)项目(2009CB522008)
NSFC-云南联合基金(U1132602)
宁波市科技创新团队(2009B21002)
宁波市医学科技计划项目(2013A26)