小麦株高相关性状与SNP标记全基因组关联分析

Genome-Wide Association Analysis between SNP Markers and Plant Height Related Traits in Wheat

株高是影响小麦产量和控制倒伏的重要因素,研究小麦株高相关性状的遗传机制对高产育种具有指导意义。以205份中国冬麦区小麦品种(系)为材料,利用分布于小麦全基因组的24 355个单核苷酸多态性(SNP)标记对株高相关性状进行关联分析。共发现38个与株高相关性状显著关联(P<0.0001)的SNP,分布在1B、2A、2B、3A、3B、3D、4A、4B、5A和6D染色体上。其中,11个位点至少在2个环境中稳定表达,可用于开发CAPS标记。同时,发掘了一批株高性状相关基因的优异等位变异,如降低株高的等位变异Bob White_c48009_52,平均降低株高12.9 cm;控制穗下节间长的等位变异BS00039422_51-C和IAAV1698-A,分别调控穗下节间长5.9 cm和6.6 cm。本研究发掘的控制小麦株高基因位点为在分子水平上研究小麦株高复杂性状提供了有价值的参考。

Plant height （PH） has great influence on yield potential and lodging occurrence in wheat （Triticum aestivumL.）. In this study, the diverse panel of 205 elite wheat lines was genotyped with the 90k SNP array （24 355 SNPs） based on the Illumina In-finium assay to disclose the PH genetic mechanism. A total of 38 SNPs were found to be associated with PH （P 〈 0.0001）, which were located on chromosomes 1B, 2A, 2B, 3A, 3B, 3D, 4A, 4B, 5A, and 6D, including 11 SNPs detected in two or more envi-ronments. These stable SNPs can be used to develop CAPS markers for PH. In which, a few elite alleles were identified, such as BobWhite_c48009_52 to reduce PH by 12.9 cm, and BS00039422_51-C and IAAV1698-A to decrease length of the first inter-node below spike by 5.9 cm and 6.6 cm, respectively. These results will facilitate further researches in PH-related traits in wheat.