还原型谷胱甘肽对急性百草枯中毒治疗作用的实验研究

Experimental Study on the Therapeutic Effect of Reduced Glutathione Against Acute Poisoning By Paraquat

目的:探讨还原型谷胱甘肽对急性百草枯中毒的治疗作用。方法:大鼠用百草枯灌胃(250 mg·kg-1)染毒后不同时间腹腔注射还原型谷胱甘肽(GSH)分别测定染毒后8 h、24 h、48 h、72 h大鼠血浆和支气管肺泡灌洗液(BALF)中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力,并观察肺组织结构改变。结果:PQ染毒后血浆及BALF中MDA显著高于对照组。给予GSH治疗后,与PQ染毒组相比,血浆和BALF中的MDA下降;SOD、GSH-Px逐渐升高。投用GSH的不同时间点之间SOD、MDA的差异不大。结论:给予还原型谷胱甘肽,可使急性百草枯中毒引起的脂质过氧化损害得到改善。

Objective： To study the therapeutic effect of reduced glutathione（ GSH） against acute poisoning by paraquat.Methods： SD rats were given GSH at different time after oral administration of paraquat（ PQ,250 mg·kg- 1）. 8 h,24 h,48 h,72 h later,the level of MDA,the activities of superoxide dismutase（ SOD） and glutathione peroxidase（ GSHPx） in plasma and bronchoalveolar lavage fluid（ BALF） of the rats were detected respectively. Results： The level of MDA of the PQ group significantly increased both in plasma and BALF（ P ﹤ 0. 01） compared with normal group. Compared GSH-treated group with PQ poisoning group,the MDA level decreased both in plasma and BALF（ P ﹤ 0. 05）,but the SOD and GSH-Px activities were increased. The administration of GSH at different time after poisoning showed a little difference in MDA level and the activities of SOD and GSH-Px. Conclusion： Administration of GSH could improve the lipid peroxidation damage caused by acute poisoning of paraquat.