3,4亚甲基二氧基甲基苯丙胺对小鼠微核率及染色体畸变率的影响

Study on micronucleus frequency and chromosomal aberration ratios of mouse testicle cells induced by methylene-dioxymethamphetamine

目的通过连续20 d对雄性小鼠灌胃染毒3,4亚甲基二氧基甲基苯丙胺(MDMA)后,探究MDMA对雄性小鼠睾丸组织细胞微核率及染色体畸变率的影响。方法将雄性小鼠随机数字表法分为MDMA低(5.0 mg/kg)、中(10.0 mg/kg)、高(20.0 mg/kg)3个染毒剂量组,采用0.9%氯化钠注射液做阴性对照,每日染毒1次。于末次给药后第2天,取小鼠睾丸组织细胞,采用常规微核(MN)试验,检测小鼠睾丸细胞微核率的改变;同时采用染色体畸变试验探究MDMA对小鼠睾丸细胞染色体畸变率的影响。结果微核试验结果表明MDMA高剂量与低剂量组小鼠睾丸细胞微核率及阴性对照组比较,差异有统计学意义(P<0.01)。染色体畸变试验结果中,MDM高、中剂量组染色体畸变率分别与阴性对照组比较,差异有统计学意义(P<0.05),高剂量组与中、低剂量组染色体畸变率比较差异有统计学意义(P<0.05)。结论高剂量组MDMA能诱导小鼠睾丸组织细胞微核率增加,高剂量组及中剂量组可以使小鼠睾丸细胞染色体畸变率增高,说明其对雄性小鼠睾丸遗传物质有一定的损伤效应。

Objective To study the effects of 3,4-methylenedioxymethamphetamine （MDMA） on micronucleus frequency and chromosomal aberration ratios of male mouse testicle cells. Methods Healthy adult male rats were randomly divided into four groups. Different doses of MDMA （0, 5, 10 and 20 mg/kg） were administrated to the rats every days for 20 days. The rats were killed after the last test substance given for 24 h. Each rat＇s micronucleus frequency and chromosomal aberration ratio of testicle cell were detected by using micronucleus test and chromosomal aberration test. Results The micronucleus frequencies of rats testicle cell in high dose group was significantly different from the control group and low dose group （P〈0.01）. With the control group, the chromosomal aberration ratios in testis of rats in high and middle dose group increased, the difference was significant （P〈0.05）, that in high dose group was also significantly different from the low and middle dose group （P〈0.05）. Conclusion It was suggested that MDMA might damage genetic material of rat testicle cells.