摘要
目的:探讨PL-11血小板分析仪在检测血小板数量及聚集功能方面的性能。方法按照美国临床实验室标准化协会( CLSI)制定的仪器性能验证标准及我国卫生行业标准 WS/T 406-2012《临床血液学检验常规项目分析质量要求》对PL-11血小板分析仪进行血小板计数及聚集功能的性能评价。采用PL-11血小板分析仪、LBY-NJ4血小板聚集仪及TEG-5000血栓弹力图仪检测健康人群血小板聚集率,分析各仪器检测结果间的相关性。结果 PL-11血小板分析仪的批内及日间精密度均<1/3总误差(7%);携带污染率为0.32%;在(4.12~1380.4)×109/L线性范围内回归方程斜率为1.03,R2=0.993;血小板计数结果与血小板参考方法的结果符合率为84%,均符合行业标准要求。分别采用PL-11血小板分析仪、LBY-NJ4血小板聚集仪及TEG-5000血栓弹力图仪检测79例2型糖尿病患者单服用氯匹格雷前、后血小板聚集率,3种仪器之间差异均无统计学意义( P>0.05),患者服药前、后血小板聚集率差异有统计学意义(P<0.01)。结论 PL-11血小板分析仪可为临床提供准确、可靠的血小板计数及聚集功能的检测结果。
Objective To investigate the performance of PL-11 platelet analyzer in the detection of platelet count and platelet aggregation.Methods According to the instrument performance verification standards in the Clinical and Laboratory Standards Institute ( CLSI) and the Health Industry Standard of the People′s Republic of China, WS/T 406-2012:the Quality Standard of Routine Tests in Clinical Hematology, the performance of PL-11 platelet analyzer in the detection of platelet count and platelet aggregation were evaluated.Platelet aggregation rate in healthy subjects was compared for correlation, which was detected by PL-11 platelet analyzer, LBY-NJ4 platelet tester and TEG-5000 thromboelastogram.Results The within-run and inter-day precisions of PL-11 platelet analyzer were〈1/3 of total error (7%), and the carry-over rate was 0.32%.In (4.12-1 380.4) ×109/L linear range, the regression equation slope was 1.03 (R2 =0.993).The coincidence rate for platelet count by PL-11 platelet analyzer with reference method was 84%.There was no statistical significance of platelet aggregation rate among PL-11 platelet analyzer, LBY-NJ4 platelet tester and TEG-5000 thromboelastogram in 79 patients with type 2 diabetes mellitus (P〉0.05).However, there was statistical significance in platelet aggregation rates before and after taking clopidogrel hydrogen(P〈0.01).Conclusions PL-11 platelet analyzer can provide accurate and reliable results for platelet count and aggregation.
出处
《检验医学》
CAS
2015年第9期926-930,共5页
Laboratory Medicine
关键词
血小板聚集
血小板分析仪
连续血小板计数
性能评价
Platelet aggregation
Platelet analyzer
Continuous platelet count
Performance evaluation