蜗牛酶促毛蕊异黄酮苷转化成毛蕊异黄酮的条件研究

Calycosin-7-O-β-D-Glucoside Transformation into Calycosin by Incubation with the Snailase

目的探讨蜗牛酶酶解毛蕊异黄酮苷转化生成毛蕊异黄酮的最佳转化条件和方法。方法采用恒温摇床，将蜗牛酶溶于合适温度的水中，加入用DMSO溶解的苷，边加边搅拌，加完后反应30min，最后用乙腈终止反应。通过反复重结晶得到所需要的苷元。结果最佳转化条件为，当蜗牛酶与毛蕊异黄酮苷的比例为1：2时，加入200mL的保温35—37℃水中，待蜗牛酶溶解均匀后，再加入毛蕊异黄酮苷（溶于1mL的DMSO中），pH维持5—8，孵化0．5h后，用乙腈等量终止反应。结论该法能有效使毛蕊异黄酮苷转化为纯度大于98％的毛蕊异黄酮，方法简单，易于操作。

Objective To study the best best conditions and methods to conduct the transformation of calycosin- 7- O- β- D- glucoside into calycosin using the enzymatic hydrolysis with snailase. Methods The snailase was dissolved in a suitable water in constant temperature shaking table and then added dimethyl sulfoxide（DMSO） which calycosin-7-O- β- D-glucoside was dissolved in, kept the reaction for 30 min, and finally terminated the reaction with acetonitrile. Results When the snailase and calycosin - 7 - O - β - D - glucoside were in the ratio of 1： 2, added with 200 mL water（keeping in 35-37 ℃ ）,added calycosin-7-O-β- D-glucoside that was dissolved in 1 mL of DMSO, and the pH was maintained at between 5- 8, then quenched with an equal amount of acetonitrile after incubating 0. 5 h. Conclusion This method can easily transform the calycosin-7- O- β- D-glucose into calycosin（ 〉 98% ）, and the operating process is simple and effective.