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农杆菌介导水稻遗传转化方法的优化研究 被引量:2

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摘要 目前人们对转基因方法的研究越来越多,其中,对农杆菌介导的转化方法研究较多,应用最广。本实验对农杆菌介导水稻遗传转化的各重要因素进行了分析,研究讨论了光照、继代次数、菌液浓度、侵染时间、干燥情况等因素对遗传转化的影响。研究结果表明,光照诱导愈伤比暗培养诱导缩短约一周的时间,菌液浓度过高易导致后期实验抑菌抑不住,侵染时间过长会导致愈伤软化,进而很难分化。
作者 周蕾 陈晨
出处 《农业与技术》 2015年第17期33-34,37,共3页 Agriculture and Technology
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