CK4、CK14在小鼠食管上皮的表达

The Expression of CK4,CK14 in the Esophageal Epithelium of Mice

为观察细胞角蛋4(CK4)和14(CK14)表达与小鼠食管上皮发育之间的关系。采用免疫组织化学观察CK4、CK14在胚胎第11天开始到胚胎第18天、小鼠出生后(生后)第1-7天、生后第2周、生后第4周以及成年小鼠食管上皮细胞的表达情况。结果显示,CK4和CK14在小鼠食管上皮均有表达,但是CK4在胚胎第15天开始表达,而CK14在胚胎第11天开始就有表达,二者表达的相同之处在于:在小鼠胚胎第16天开始表达明显,且定位明确,以后随着胚胎发育,表达逐渐增强,但在生后第7天表达最强,其中CK4主要在食管上皮基底层以上表达,而CK14在食管上皮的基底层表达,随后在生后第2周、第4周和成年的表达又逐渐降低。由此可知:CK14可以结合干细胞标记物,共同标记食管上皮干细胞。

To study the relation between the CK4 and CK14 expression and the development of esophageal epithelium of mice. The CK4 and CK14 immuno-positive cells were observed by immnnohistochemical method during El ld to E18d and 1st day to 7th day,14th day to 28th day after birth.Results：The expression of both CK4 and CK14 were observed in esophageal epithelium of mice. But the CK4 expression was firstly observed at E15d,and CK14 at Elld,both of which showed the correct location at E16,then continued to rise until they were significantly higher in the esophageal epithelium of mice in the 1st -week after birth.The expression of CK4 was located in the suprabasal layer,while the CK14 in the basal layer.Then the expression of both protein decreased in the second week,the fourth week after birth and the adult.It is concluded that the CK14 can be used to locate the situation of stem cells in esophageal epithelium.