靛蓝在虹鳟鱼体内定位分布的放射自显影研究

Autoradiographic Disposition of Indigo in Rainbow Trout

摘要：以虹鳟鱼为实验鱼类，通过水浴实验，以CYP1诱导剂（PCB126）为研究对象，分析了靛蓝在鱼全身代谢的动力学情况。通过放射自显影方法并利用放射性^14C-标记的靛蓝（18nM）进行水浴暴露实验，结果表明：实验开始20min后靛蓝消化于胆液中；在随后的实验中（暴露3—24h），在胆汁的排泄途径中发现高浓度的标记物。在鱼鳃中也存在高浓度的标记物；换水后，其放射性浓度逐渐降低。同时观察到嗅觉器官中有选择性的标记，肾脏和肝脏中吸收的放射性物质浓度低于鳃。比较暴露于二甲基亚砜（DMSO）和CYP1诱导物PCB126的鱼。其^14C-靛蓝总体布局模式相似。CYP1抑制剂玫瑰树碱可以阻拦^14C-靛蓝在鱼鳃中的积蓄。

In the study, the whole-body kinetics of indigo in rainbow trout was examined through exposure via ambient water. Determined by autoradiography, there was an uptake of ^14 C-indigo （18 nM） from the water and a subsequent elimina- tion into the bile within 20 mins. At later time-points （3-24 h of exposure; 24 h of exposure plus 1-2days in clean water）, high concentration ^14C labelled substances were present in the biliary excretory pathways. A high concentration of radioactivity appeared in the gill filaments throughout the exposure period （24 h）, and the concentration gradually decreased when fish were put in clean water for depuration. A selective labelling of the olfactory organ was also observed. The uptake of radioac- tivity in the kidney and liver was pronounced but lower than in the gills. The overall distribution pattern of ^14 C-indigo was similar in fish exposed to the vehicle （DMSO） and in fish exposed to the CYP1 inducer, PCB126. The accumulation of ^14 C- indigo in the gills was, however completely blocked in PCB 126-exposed fish exposed to the CYP1 inhibitor ellipticine.