实验性自身免疫性脑脊髓炎(EAE)C57BL/6J小鼠模型构建

Establishment of the experimental autoimmune encephalomyelitis( EAE)model in C57BL/6J mice

目的建立慢性-非缓解的实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)模型,以便用于多发性硬化症的研究和治疗。方法采用背部多点MOG35-55免疫建立EAE模型,模型期间按Benson评分标准对EAE的病程进行临床评估,取发病高峰期脊髓和脑组织进行H&E染色,观察炎症细胞浸润情况。结果经该方法建立C57BL/6J小鼠EAE模型,具有稳定、发病率高的特点。对EAE小鼠的脊髓、脑组织进行的病理学检测结果表明,与对照组小鼠相比,EAE小鼠的脊髓、脑组织中炎性细胞浸润增多。结论本研究成功构建了MOG35-55免疫的C57BL/6J EAE小鼠模型。

Objective To obtain chronic progressive EAE model used to study the pathogenesis and identify the novel drug target of multiple sclerosis. Methods The C57BL/ 6J mice were immunized with immunogen MOG35 - 55 by multi - point subcutane-ous injection in the hind flank. The clinical score assessment was performed daily according to Benson’s scoring criteria. And the histological examination of H＆E - stained paraffin - embedded sections of spinal cord and brain harvested at peak of EAE was performed. Results A stable EAE model was successfully estab-lished with high incidence. Compared with the normal mice,inflammatory cell infiltrates were increased in the spinal cord and brain tissue of EAE mice. Conclusion We successfully established the MOG35 - 55 - induced EAE model in C57BL/ 6J mice.