米非司酮(RU486)、ZK98.734对离体培养人蜕膜细胞形态的影响

THE EFFECT OF THE ANTIPROGESTINS RU486 AND ZK98.734 ON THE MORPHOLOGY OF HUMAN DECIDUAL CELLS IN CULTURE

本文采用人早孕蜕膜细胞离体培养的方法,观察不同浓度的抗孕激素RU486(米非司酮)和ZK98.734 对蜕膜细胞生长发育的作用。结果表明,(1)在浓度为5×10^(-4)mol/l—5×10^(-6)mol/l的范围内,RU486(米非司酮)和ZK98.734对离体培养人蜕膜细胞生长发育具有抑制作用,作用的强度与浓度有关。(2)两种抗孕激素相比较,RU486的作用强于ZK98.734。(3)抗孕激素对蜕膜细胞的作用是暂时的、可恢复的。研究结果提示,抗孕激素除通过受体途径达到抗早孕目的外,对蜕膜组织的生长发育也有抑制作用,但由于这一作用具有可逆性,故只能在某一时期发挥作用。

The effects of RU486 and ZK98. 734 on the morphology of humandecidual cells (DE) were studied. Decidua obtained from women in earlypregnancy (45-50 days amenorrhea from the last menstrual period) weregrown in tissue culture.Both RU486 and ZK98. 734 could inhibit the proli-feration and differentiation of DE. The morphological changes of DEinduced by the antiprogesti ns were dose-and time-dependent within theconcentration range from 5× 10^(-4)mol/l to 5 ×10^(-6)mol/l. Their inhibitoryeffect was reversible after the removal of the drugs. The effect of RU486is slightly stronger than that of ZK98. 734 at the same dosage(5× 10^(-5)mol/l).It is concluded that the development of human DE in culture may beaffected directly by the two antiprogestins and their effect is reversible.