摘要
本研究从黑龙江省某养鹅场送检的小鹅瘟疑似病例中分离到一病原,采用鹅胚接种、PCR检测、IFA检测、病毒效价测定和动物致病性试验等方法对该病原进行鉴定。结果显示,所分离到的病原为鹅细小病毒(GPV),将其命名为YG株;YG株的TCID50为104.0/0.2mL;对YG株全基因组进行扩增和序列分析后发现,YG株与标准毒株B株的核苷酸同源性最高,高达98.3%;病理学观察显示,GPV对感染后的雏鹅具有明显的组织损伤,主要侵害易感雏鹅的消化系统;免疫组织化学法检测结果表明,抗原主要分布在雏鹅的小肠、肝、肾及脾中,其中小肠中的抗原含量最高。
A pathogenic agent was isolated from goslings with pathogenic changes and clinical symptoms of Derzsy＇s disease from Heilongjiang Province.Indirect immunofluorescence assay,PCR,and sequence results confirmed that the pathogen might be goose parvovirus（GPV）,named as YG strain.The virus titer of YG strain was 104.0/0.2mL in TCID50.Sequence analysis indicated that the full genome of YG strain showed 98.3% homology to B strain.The YG infected birds mainly showed the pathological damage in their digestive system.Immunohistochemistry assay confirmed that GPV antigen located mainly in the small intestine,livers,kidneys,and spleens,of which the antigen in small intestine with the highest quantity.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2015年第9期912-917,共6页
Chinese Veterinary Science
基金
现代农业水禽产业技术体系岗位科学家专项(CARS-43-10)
国家自然科学基金面上项目(31072132)
关键词
鹅细小病毒
分离鉴定
器官分布
goose parvovirus
isolation and identification
distribution in organs
