大蒜素标准品DATS体外抗粪肠球菌生物膜作用的研究

Effects of Standard Allicin on Enterococcus Faecalis Biofilms Control:an in vitro Study

目的:探讨不同浓度大蒜素对照品二烯丙基化三硫(dially trisulfide,DATS)体外对粪肠球菌生物膜的作用。方法:体外培养形成粪肠球菌生物膜,分别经1280、2560、5120mg/L浓度的DATS作用后,采用MTT法观察DATS对生物膜粪肠球菌的抑菌率。结果:1280 mg/L DATS对对数期和饥饿期的生物膜粪肠球菌抑菌率约71%,显著高于对稳定期生物膜粪肠球菌的抑菌率58%,差异具有统计学意义(P<0.05),随着药物浓度的增高,抑菌率增高。结论:DATS对生物膜中的粪肠球菌具有明显杀灭或抑制作用。

Objective： To investigate the antibacterial efficacy of different concentrations dially trisulfide （DATS） on the biofilms of Enterococcus faecalis （E. faecalis） in vitro. Methods： E. faecalis was cultured in vitro to form the bacterial biofilm of different growth periods. Different concentrations of DATS, i.e. 1280μg/mL, 2560μg/mL and 5120μg/mL, were added to E. faecalis biofilms in different growth periods, and the bacteriostasis rates were measured by MTT method. Results： The bacteriostatic rates of 1280μg/mL DATS were about 71% in logarithmic phase and hunger phase, which were significantly higher than that in stability phase with bacteriostasis rate of 58 % （P〈 0.05）. DATS in concentration of 2560μg/mL and 5120μg/mL showed better antibacterial efficacy than that of 1280μg/mL, indicating that the antibacterial efficacy of DATS was in a concentration--dependent manner （P〈0. 05）. Conclusion： DATS played a role in suppressing E. faecalis in the biofilm in vitro .