静态张应力作用下人牙周膜成纤维细胞β-catenin和Wnt3a蛋白的表达

Expression of β-catenin and Wnt3a under Static Tensile Stress in Human Periodontal Ligament Fibroblasts

目的:研究人牙周膜成纤维细胞在不同大小机械张应力作用下Wnt/β-catenin信号通路中β-catenin和Wnt3a蛋白的表达变化。方法:运用酶解组织块法体外培养人牙周膜成纤维细胞,将细胞按加力时间的不同(2h、4h、6h)分为3组,每组再按形变率不同将其分为8%、12%和16%3个亚组,并取加力时间0h、形变率0%作为对照组。用western blot技术检测细胞在不同时间点和不同形变率作用下Wnt/β-catenin信号通路中β-catenin和Wnt3a蛋白的表达变化。结果:Western blot结果显示,人牙周膜成纤维细胞在机械力加载后β-catenin和Wnt3a蛋白表达显著减少(P<0.05)。在加力时间相同的组内(2h、4h、6h),β-catenin和Wnt3a蛋白表达随着形变率的增大而逐渐减少(P<0.05)。结论:β-catenin和Wnt3a参与了静态张应力作用下牙周膜成纤维细胞的代谢,机械力对牙周膜成纤维细胞中Wnt/β-catenin信号通路有抑制作用。

Objective： To investigate the effects of different static tensile stress on β-catenin and Wnt3a expression of Wnt/β-catenin signaling pathway in human periodontal ligament cells （HPLFs）. Methods： HPLFs were cultured by tissue enzyme digestion method in vitro. They were divided into three groups according to loading time for 2h, 4h and 6h. Then each group was divided into three subgroups according to static tensile stress of 8%, 12% and 16 % elongation. And the group without loading or elongation was set as control group. The expressions of β- catenin and Wnt3a were detected by western blot analysis. Results： Western blot analysis showed that static tensile stress could significantly reduce the expression of β-catenin and Wnt3a in HPLFs when compared with the control group （P〈0.05）. And in each subgroup, the expression of β-catenin and Wnt3a were decreased with gradually increased static tensile stress （P〈0.05）. Conclusion： β-catenin and Wnt3a participated in the metabolism of HPLFs which can be inhibited by static tensile stress.