橙皮素的大鼠血浆蛋白结合率研究

Study on plasma protein binding rate of hesperetin in rat' plasma

目的测定橙皮素的大鼠血浆蛋白结合率。方法采用平衡透析法结合高效液相色谱法(HPLC)对橙皮素与大鼠血浆的血浆蛋白结合率进行测定。结果在0.5、2.5和10μg/m L浓度水平橙皮素与大鼠血浆的血浆蛋白结合率分别为(93.5±4.2)%,(94.3±5.6)%和(93.9±3.8)%,三个浓度水平之间的血浆蛋白结合率差异无统计学意义(P≥0.05)。结论橙皮素与大鼠血浆蛋白高度结合。

Objective To determine the rats′ plasma protein binding rate of hesperetin. Methods The equi-librium dialysis method combined with HPLC was used to determine the plasma protein binding rate of hesperetinin rats. Results The plasma protein binding rates of hesperetin in rats at the concentration of 0. 5,2. 5 and 10 μg/mL were （93. 5 ± 4. 2）%,（94. 3 ± 5. 6）% and （93. 9 ± 3. 8）% respectively,and there was no significant difference among these concentration levels（P≥0. 05）. Conclusion Hesperetin is highly bound to rats′ plasma protein.