铜离子胁迫对小球藻生物量、蛋白质、多糖及MDA含量的影响

Effect of copper ions on Chlorella vulgaris biomass,protein,polysaccharide and MDA content

用不同浓度Cu2＋（0，0.1，0.4，0.7 mmol？L －1）处理小球藻，通过测定其生物量、蛋白质、多糖以及丙二醛（malondialdehyde ，MDA）含量来反映小球藻的生长状况．结果表明，在不同浓度Cu2＋的胁迫下，0～2 d高浓度Cu2＋促进小球藻的生长，2～6 d则表现为低浓度促进小球藻的生长，而高浓度抑制其生长．蛋白质含量随着C u2＋浓度的增加而明显降低，说明低浓度的C u2＋促进而高浓度C u2＋抑制小球藻合成蛋白质．在C u2＋胁迫下小球藻多糖明显高于对照组，但随着Cu2＋浓度的增加，含量依次降低． MDA含量在无Cu2＋影响下，无明显的变化，在0.7 mmol？L －1时， MDA含量最高．

Different concentrations of Cu2＋ （0 ,0.1 ,0.4 ,0.7 mmol-L -1 ） are used for processing of Chlorella vulgaris ,and the growing status of Chlorella vulgaris is reflected by determining the biomass , protein , polysaccharide and MDA （malondialdehyde , MDA ） content . The results show that ：at the different concentrations of Cu2＋ stress , high concentration of Cu2＋ can promote the grow th of Chlorella vulgaris in 0 ~ 2 d . Howevr , low concentration can promote the growth , while high concentration of Cu2＋ can inhibit growth in 2~6 d . Protein content decreases significantly with increasing of Cu2＋ , this indicates that protein synthesis is promoted in low concentration of Cu2＋ and inhibited in high concentration of Cu2＋ . Polysaccharide is significantly higher than the control group in the stress of Cu2＋ , while polysaccharide content increases successively with increasing of Cu2＋ .MDA content is the highest in 0.7 mmol-L -1 ,while without affecting Cu2＋ ,and MDA content has no obviously changes .