大豆总皂甙对大强度运动大鼠股四头肌自由基代谢和血清睾酮及LDH、BUN的影响

Effects of Total Soy Saponins on Free Radicals,Serum Testosterone,LDH and BUN in the Quadriceps Femoris of Exhausted Rats

实验动物分为四组:对照组、安静皂甙组、力竭组、力竭皂甙组.安静皂甙组和力竭皂甙组按照20mg/kg体重经口腔给药,每日一次,持续2周.力竭组灌服相同剂量生理盐水,对照组不给予任何处理.2周后进行一次跑台急性力竭运动.动物力竭后迅速断头处死,测定股四头肌SOD、CAT、MDA、GSH-Px、GR、GSH、T-AOC含量及血清BUN、LDH、睾酮水平.结果显示:1.大豆总皂甙(TS)能显著延长大鼠力竭运动时间;2.TS能显著提高其血睾酮水平同时降低血清BUN水平;3.TS对运动力竭大鼠股四头肌各抗氧化指标显示出了正性的影响,尤其是提升T-AOC和降低MDA,这可能与TS的多种生物学作用有关.

All rats were divided into four groups：Control group,TS group,exhausted group and TS exhausted group. TS group and TS exhausted group were gavaged with TS through the mouth with a dosage of 20mg/kg body weight,once a day for 2 weeks. Exhausted group were given placebo. Control group were not given any treatment. Treadmill speed was set at 30m/min, and the rats（Exhausted group and TS exhausted group）were trained at this speed until exhaus- ted. The exercise time and exercise distance were recorded when the rats exhausted, and then the rats were decapitated and anatomized immediately. 10% homogenate of quadriceps femoris tissue was prepared. The indicators of superoxide dismutase（SOD）,catalase（CAT）,malondialdehyde （MDA）, glutathione peroxidase （GSH-Px）, glutathione reductase （GR）, glutathione （GSH）, total an- tioxidant capacity（T-AOC）and LDH were tested by spectrophotometry. BUN was tested by Hitachi 7060 automatic biochemical analyzer. Serum testosterone was tested by radioimmunoassay. The results show that：l. TS can significantly increase the rats exercise time;2. TS can significantly in- crease the rat＇s testosterone and decrease serum BUN;3. TS has a positive effect on the anti-oxidative indexes of quadriceps femoris in exhaustive exercised rats,especially increase T-AOC and decrease MDA.