摘要
对碳纳米管(CNTs)进行酸化处理,采用乳化交联法制备CNTs/壳聚糖(CS)复合微球,在其表面诱导羟基磷灰石仿生合成,研究了CNTs对复合微球仿生矿化的影响,并与纯CS微球的仿生矿化进行了对比.利用扫描电子显微镜(SEM)、X射线衍射仪(XRD)、溶胀率和含水率测试等考察了复合微球矿化前后的形貌特征、物相结构及稳定性.结果表明,在相同时间下,CNTs/CS复合微球表面纳米羟基磷灰石的形成能力明显优于纯CS微球,且形态稳定性更高.细胞实验结果表明,与MG63细胞共培养7 d时,矿化复合微球细胞增殖明显.
Carbon nanotubes / chitosan( CNTs / CS) composite microspheres were prepared by emulsification and cross-linking method. Nano-hydroxyapatite crystals were biomimetically formed on the surface of the composite microspheres. The influence of CNTs on the mineralization of the microspheres was investigated. The morphologies,structurs and stability properties of the microspheres before and after mineralization were studied through scanning electronic microscopy( SEM),X-ray diffraction( XRD),swelling ratio and water content tests. The results show that the formation ability of the nano-hydroxyapatite on the CNTs / CS microspheres is significantly higher than that on pure CS microspheres. And the mineralized CNTs / CS microspheres exhibit better structure stability. After culturing with MG63 cells for 7 d,the proliferation of cells cultured with mineralized CNTs / CS microspheres shows significant difference from that of the cells cultured with mineralized CS microspheres.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2015年第10期1873-1879,共7页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:11502158,31271005,21201142,51402207)
山西省青年科技基金(批准号:2013021014-2)
山西省高等学校科技创新基金(批准号:2013111)资助~~
关键词
碳纳米管
壳聚糖
复合微球
原位矿化
细胞响应
Carbon nanotube
Chitosan
Composite microsphere
In situ mineralization
Cellular response