UPLC-MS/MS同时测定双黄连片中5种有效成分的含量

Simultaneous Determination of Five Effective Components in Shuanghuanglian Tablet by UPLC-MS / MS

目的：建立同时测定双黄连片中5种有效成分含量的UPLC-MS/MS分析方法。方法：采用BEHC18色谱柱（2.1mm×100 mm,1.7μm）,流动相乙腈-0.1%甲酸水梯度洗脱,流速0.4 m L·min-1,采用电喷雾电离源（ESI）,多反应离子监测（MRM）扫描方式。结果：10种被测物呈良好的线性关系（r〉0.999）;精密度、重复性和稳定性良好;加样回收率在96.00%~104.67%,RSD均〈3%。结论：所建立的方法准确、快捷,重复性好,可用于双黄连片中绿原酸、连翘苷、连翘酯苷A、木樨草苷、芦丁5种有效成分的含量的同时测定。

Objective： To establish a HPLC-MS/MS method for simultaneously determining five components （ehronogenic acid, phillyrin, forsythoside A, luteoloside, rutin ） in Shuanghuanglian tablets. Method： Waters Acquity BEH Clscolumn （2.1 mm × 100 mm, 1.7 μm） was adopted and eluted with the mobile phase of acetonitrile and 0. 1% formic acid/water in a gradient mode at a flow rate of 0.4 mL .min-1 The analytes were detected by tandem mass spectrometry with the electrospray ionization （ESI） source in the multiple reaction monitoring （MRM） mode. Result： Ten compounds showed a good linear relationship （r 〉 0.999）; the precisions, repeatabilities and stabilities of the method were good, and the average recoveries were between 96% and 104.67% with the relative standard deviations of no more than 3%. Conclusion： The established method is highly repeatable, rapid and accurate and can be used for the determination of five effective components in Shuanghuanglian tablets.