不同产地马齿苋HPLC指纹图谱

HPLC Fingerprints of Portulaca oleracea from Different Places

目的:建立马齿苋水提液HPLC特征指纹图谱。方法:采用回流提取制备不同产地马齿苋药材供试品溶液,流动相乙腈-甲醇(7∶3)-0.1%甲酸水溶液梯度洗脱,检测波长320 nm,建立马齿苋水提液的HPLC指纹图谱,并进行相似度评价。结果:10种不同产地马齿苋供试品溶液指纹图谱中确定了11个共有峰,5号峰咖啡酸为参照峰,10批样品指纹图谱中12个共有峰的相似度均>0.80。结论:该方法具有较好的重复性和稳定性,所建立的马齿苋HPLC指纹图谱标定了11个共有峰,色谱峰的重叠率较高,说明马齿苋没有明显的地域差异性,该方法将为马齿苋药材质量控制提供可靠依据,为其相关及临床应用研究提供借鉴。

Objective： To establish an HPLC fingerprint method of water extracts from Portulaca oleracea. Method： The sample solutions of P. oleracea from different origins were prepared by the reflux extraction. An HPLC fingerprint analysis was performed with acetonitrile-methanol （7： 3） and 0.1% formic acid as the mobile phase by gradient elution. The UV detection was 320 nm. And the similarity evaluation analysis was conducted. Result： Totally 11 common peaks were identified in the fingerprints of sample solution of 10 types of P. oleracea from different origins. With the No. 5 peak caffeic acid as the reference peak, the similarities of 12 common peaks in the fingerprints of the 10 batches of samples were more than 0.80. Conclusion： The method has a good reproducibility and stability and identify 11 common peaks in the HPLC fingerprint, with a high overlap rate in the chromatographic peaks, indicating no significant regional differences in P. oleracea. It can provide a reliable basis for the quality control of P. oleracea and a reference for relevant and clinic application studies.